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Recombinant fragment corresponding to Human MiTF (N terminal).
This antibody clone is manufactured by Abcam.We can conjugate this antibody to FITC for you (please see ab150195 for details). Do not store in a frost-free freezer. The antibody is stable for at least 12 months when stored appropriately. Working dilutions should be discarded if not used within 12 hours.
Our Abpromise guarantee covers the use of ab12039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 1 µg/ml. Antigen retrieval is not essential but may optimise staining.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 16648630
Fix with methanol/acetone, block with 1% BSA/PBS (see Wellbrock et al).
|ChIP||Use at an assay dependent concentration. PubMed: 21990371|
|EMSA||Use at an assay dependent concentration.|
|Flow Cyt||Use 2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 52-56 kDa (predicted molecular weight: 59 kDa).|
|IP||Use at 2 µg/mg of lysate.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"