HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded) with ab70843 at 1 µg/ml for WB, subsequent to being used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western blot - Anti-MIS12 antibody (ab70843)
All lanes : Anti-MIS12 antibody (ab70843) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling MIS12 with ab70843 at 1/1000 (1µg/ml). Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-MIS12 antibody (ab70843)This image is courtesy of an abreview by Eleni Petsalaki.
ab70843 staining MIS12 in a human colon carcinoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and incubated with primary antibody (1/100 in PBS + 1% BSA) for 1 hour. An undiluted FITC-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.