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Aldosterone 3. This antibody was produced using the anti idiotypic method.
Our Abpromise guarantee covers the use of ab2774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/20. PubMed: 20573992
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|Inhibition Assay||Use at an assay dependent concentration.
ab2774 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||1/100. Immunohistochemical staining of MR in rabbit atrium with ab2774 results in strong staining of myocytes and endothelial cells. In immunohistochemical studies, staining with ab2774 is blocked by pre incubating the sample with aldosterone.|
|WB||1/250. Predicted molecular weight: 107 kDa. By Western blot, this antibody detects a 116 kDa protein representing MR in rabbit heart homogenate. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence.|
Formalin-fixed, paraffin-embedded cow kidney tissue stained for Mineralocorticoid Receptor using ab2774 at 1/200 dilution in immunohistochemical analysis.
Heat mediated Antigen retrieval using Citrate buffer, 10 mM pH 6.0 was used.
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