製品の概要
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製品名Anti-Mineralocorticoid Receptor antibody
Mineralocorticoid Receptor 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Mineralocorticoid Receptor
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Rabbit, Horse, Cat, Dog
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免疫原
Synthetic peptide corresponding to a region within N terminal amino acids 1-50 of Human Mineralocorticoid Receptor (NP_000892)
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ポジティブ･コントロール
- HT1080 cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab97834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 107 kDa. Optimal concentration of antibody depends the protein level in a given sample. Our recommended concentrations are the starting point for general use. Customers are advised have to do a series of titration tests to determine optimal concentration. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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機能Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
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組織特異性Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
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関連疾患Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion. -
配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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翻訳後修飾Phosphorylated.
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細胞内局在Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 475461 Dog
- Entrez Gene: 4306 Human
- Omim: 600983 Human
- SwissProt: P08235 Human
- Unigene: 163924 Human
- Unigene: 723668 Human
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別名
- Aldosterone receptor antibody
- MCR antibody
- MCR_HUMAN antibody
see all
Anti-Mineralocorticoid Receptor antibody 画像
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Western blot - Mineralocorticoid Receptor antibody (ab97834)Anti-Mineralocorticoid Receptor antibody (ab97834) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 107 kDa
プロトコール
Anti-Mineralocorticoid Receptor antibody (ab97834) 使用論文
ab97834 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"