Anti-Mineralocorticoid Receptor 抗体 (ab62532)

製品の概要

  • 製品名Anti-Mineralocorticoid Receptor antibody
    Mineralocorticoid Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Mineralocorticoid Receptor
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Pig, Spider Monkey
  • 免疫原

    A region within synthetic peptide: KIRRKNCPAC RLQKCLQAGM NLGARKSKKL GKLKGIHEEQ PQQQQPPPPP, corresponding to internal sequence amino acids 649-698 of Human Mineralocorticoid Receptor

  • ポジティブ・コントロール
    • A172 cell lysate. Human kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 107 kDa).Can be blocked with Human Mineralocorticoid Receptor peptide (ab87358). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
  • 組織特異性Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
  • 関連疾患Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
    Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻訳後修飾Phosphorylated.
  • 細胞内局在Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Aldosterone receptor antibody
    • MCR antibody
    • MCR_HUMAN antibody
    • MGC133092 antibody
    • Mineralocorticoid receptor antibody
    • MLR antibody
    • MR antibody
    • NR3 C2 antibody
    • NR3C2 antibody
    • NR3C2 protein antibody
    • Nuclear receptor subfamily 3 group C member 2 antibody
    see all

Anti-Mineralocorticoid Receptor antibody 画像

  • Anti-Mineralocorticoid Receptor antibody (ab62532) at 1.25 µg/ml + A172 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 107 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 8%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Mineralocorticoid Receptor with ab62532 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-Mineralocorticoid Receptor antibody (ab62532) 使用論文

ab62532 has not yet been referenced specifically in any publications.

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