製品の概要
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製品名Anti-Mineralocorticoid Receptor antibody
Mineralocorticoid Receptor 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Mineralocorticoid Receptor
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アプリケーション適用あり: WB, IHC-Pmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Pig, Spider monkey
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免疫原
A region within synthetic peptide: KIRRKNCPAC RLQKCLQAGM NLGARKSKKL GKLKGIHEEQ PQQQQPPPPP, corresponding to internal sequence amino acids 649-698 of Human Mineralocorticoid Receptor
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ポジティブ･コントロール
- A172 cell lysate. Human kidney tissue.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab62532 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1.25 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 107 kDa).Can be blocked with Human Mineralocorticoid Receptor peptide (ab87358). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. | |
| IHC-P | Use a concentration of 4 - 8 µg/ml. |
ターゲット情報
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機能Receptor for both mineralocorticoids (MC) such as aldosterone and glucocorticoids (GC) such as corticosterone or cortisol. Binds to mineralocorticoid response elements (MRE) and transactivates target genes. The effect of MC is to increase ion and water transport and thus raise extracellular fluid volume and blood pressure and lower potassium levels.
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組織特異性Ubiquitous. Highly expressed in distal tubules, convoluted tubules and cortical collecting duct in kidney, and in sweat glands. Detected at lower levels in cardiomyocytes, in epidermis and in colon enterocytes.
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関連疾患Defects in NR3C2 are a cause of autosomal dominant pseudohypoaldosteronism type I (AD-PHA1) [MIM:177735]. PHA1 is characterized by urinary salt wasting, resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal dominant form that is mild, and the recessive form which is more severe and due to defects in any of the epithelial sodium channel subunits. In AD-PHA1 the target organ defect is confined to kidney. Clinical expression can vary from asymptomatic to moderate. It may be severe at birth, but symptoms remit with age. Familial and sporadic cases have been reported.
Defects in NR3C2 are a cause of early-onset hypertension with severe exacerbation in pregnancy (EOHSEP) [MIM:605115]. Inheritance is autosomal dominant. The disease is characterized by the onset of severe hypertension before the age of 20, and by suppression of aldosterone secretion. -
配列類似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメインComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
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翻訳後修飾Phosphorylated.
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細胞内局在Cytoplasm. Nucleus. Endoplasmic reticulum membrane. Cytoplasmic and nuclear in the absence of ligand; nuclear after ligand-binding. When bound to HSD11B2, it is found associated with the endoplasmic reticulum membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 374131 Chicken
- Entrez Gene: 4306 Human
- Entrez Gene: 110784 Mouse
- Entrez Gene: 397452 Pig
- Entrez Gene: 25672 Rat
- Omim: 600983 Human
- SwissProt: P08235 Human
- SwissProt: Q8VII8 Mouse
see all -
別名
- Aldosterone receptor antibody
- MCR antibody
- MCR_HUMAN antibody
see all
Anti-Mineralocorticoid Receptor antibody 画像
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Western blot - Mineralocorticoid Receptor antibody (ab62532)Anti-Mineralocorticoid Receptor antibody (ab62532) at 1.25 µg/ml + A172 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 107 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Gel concentration: 8% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mineralocorticoid Receptor antibody (ab62532)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Mineralocorticoid Receptor with ab62532 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
Anti-Mineralocorticoid Receptor antibody (ab62532) 使用論文
ab62532 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"