製品の概要

  • 製品名Anti-MINA53 antibody
    MINA53 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MINA53
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cat, Pig
  • 免疫原

    Synthetic peptide: MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK , corresponding to amino acids 1-50 of Human MINA53

  • ポジティブ・コントロール
    • HepG2 cell lysate; human intestine tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 30, 45 kDa (predicted molecular weight: 32, 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
  • 組織特異性Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
  • 配列類似性Belongs to the MINA53/NO66 family.
    Contains 1 JmjC domain.
  • 細胞内局在Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 60S ribosomal protein L27a histidine hydroxylase antibody
    • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
    • FLJ14393 antibody
    • Histone lysine demethylase MINA antibody
    • MDIG antibody
    • MINA antibody
    • MINA_HUMAN antibody
    • Mineral dust-induced gene protein antibody
    • Myc induced nuclear antigen 53 kDa antibody
    • MYC induced nuclear antigen antibody
    • MYC-induced nuclear antigen antibody
    • NO52 antibody
    • Nucleolar protein 52 antibody
    • Ribosomal oxygenase MINA antibody
    • ROX antibody
    see all

Anti-MINA53 antibody 画像

  • Anti-MINA53 antibody (ab50908) at 1.25 µg/ml + HepG2 cell Lysate (10µg)

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32, 53 kDa
    Observed band size : 30,45 kDa (why is the actual band size different from the predicted?)
  • ab50908 at a concentration of 4-8µg/ml positively labelling epithelial cells of human intestinal villus(indicated by arrows) in paraffin-embedded tissue. Magnification 400X
  • Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-MINA antibody (ab50908)

Anti-MINA53 antibody (ab50908) 使用論文

ab50908 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 100C, 20 minutes
Sample Human Tissue sections (colon cancer)
Specification colon cancer
Permeabilization No
Fixative Formaldehyde
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投稿 May 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"