製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MINA53
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cat, Pig
  • 免疫原

    Synthetic peptide: MPKKAKPTGS GKEEGPAPCK QMKLEAAGGP SALNFDSPSS LFESLISPIK , corresponding to amino acids 1-50 of Human MINA53

  • ポジティブ・コントロール
    • HepG2 cell lysate; human intestine tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab50908 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.25 µg/ml. Detects a band of approximately 30, 45 kDa (predicted molecular weight: 32, 53 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles.
  • 組織特異性
    Expressed in liver, skeletal muscle, heart, pancreas, and placenta. Not detected in brain, lung or kidney. Expressed in several lung cancer tissues, but is barely detected in the adjacent non-cancerous tissues. Also highly expressed in several esophageal squamous cell carcinoma (ESCC), and colon cancer tissues, and in various cancer cell lines.
  • 配列類似性
    Belongs to the MINA53/NO66 family.
    Contains 1 JmjC domain.
  • 細胞内局在
    Nucleus. Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 60S ribosomal protein L27a histidine hydroxylase antibody
    • Bifunctional lysine specific demethylase and histidyl hydroxylase MINA antibody
    • FLJ14393 antibody
    • Histone lysine demethylase MINA antibody
    • MDIG antibody
    • MINA antibody
    • MINA_HUMAN antibody
    • Mineral dust-induced gene protein antibody
    • Myc induced nuclear antigen 53 kDa antibody
    • MYC induced nuclear antigen antibody
    • MYC-induced nuclear antigen antibody
    • NO52 antibody
    • Nucleolar protein 52 antibody
    • Ribosomal oxygenase MINA antibody
    • ROX antibody
    see all

Anti-MINA53 antibody 画像

  • Anti-MINA53 antibody (ab50908) at 1.25 µg/ml + HepG2 cell Lysate (10µg)

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32, 53 kDa
    Observed band size : 30,45 kDa (why is the actual band size different from the predicted?)
  • ab50908 at a concentration of 4-8µg/ml positively labelling epithelial cells of human intestinal villus(indicated by arrows) in paraffin-embedded tissue. Magnification 400X
  • Immunohistochemistry of Human kidney lysate tissue at an antibody concentration of 4-8µg/ml using anti-MINA antibody (ab50908)

Anti-MINA53 antibody (ab50908) 使用論文

ab50908 has not yet been referenced specifically in any publications.

Product Wall

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH8, 100C, 20 minutes
Sample
Human Tissue sections (colon cancer)
Specification
colon cancer
Permeabilization
No
Fixative
Formaldehyde
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投稿 May 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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