Anti-Milk Fat Globule 1 抗体 [SPM291] (ab17787)


  • 製品名Anti-Milk Fat Globule 1 antibody [SPM291]
    Milk Fat Globule 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [SPM291] to Milk Fat Globule 1
  • 特異性We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • アプリケーション適用あり: Flow Cyt, ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    The details of the immunogen for this antibody are not available.

  • ポジティブ・コントロール
    • SKBR-3 cells. Breast, colon, or lung tissues.



Our Abpromise guarantee covers the use of ab17787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40-45 kDa (predicted molecular weight: 40-45 kDa).
IP Use at 2 µg/mg of lysate.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • 機能Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
    Medin is the main constituent of aortic medial amyloid.
  • 組織特異性Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
  • 配列類似性Contains 1 EGF-like domain.
    Contains 2 F5/8 type C domains.
  • 翻訳後修飾Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
  • 細胞内局在Membrane. Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BA46 antibody
    • Breast epithelial antigen BA46 antibody
    • EDIL1 antibody
    • HMFG antibody
    • hP47 antibody
    • HsT19888 antibody
    • Lactadherin antibody
    • Medin antibody
    • MFG-E8 antibody
    • MFGE8 antibody
    • MFGM antibody
    • MFGM_HUMAN antibody
    • Milk fat globule EGF factor 8 antibody
    • Milk fat globule EGF factor 8 protein antibody
    • Milk fat globule-EGF factor 8 antibody
    • O acetyl disialoganglioside synthase antibody
    • OAcGD3S antibody
    • SED1 antibody
    • SPAG10 antibody
    • Sperm associated antigen 10 antibody
    • Sperm surface protein hP47 antibody
    see all

Anti-Milk Fat Globule 1 antibody [SPM291] 画像

  • ab17787 Milk Fat Globule Membrane Protein staining in human tissue. The primary antibody was diluted 1/50, and incubated for 30 minutes at room temperature.
  • IHC image of ab17787 staining in human normal breast formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab17787, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Overlay histogram showing MCF-7 cells stained with ab17787 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab17787, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Milk Fat Globule 1 antibody [SPM291] (ab17787) 使用論文

This product has been referenced in:
  • Soki FN  et al. Polarization of Prostate Cancer-associated Macrophages Is Induced by Milk Fat Globule-EGF Factor 8 (MFG-E8)-mediated Efferocytosis. J Biol Chem 289:24560-72 (2014). IHC-P ; Human . Read more (PubMed: 25006249) »
  • Yu L  et al. Tumor necrosis factor a up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor ?B activation, resulting in cell migration of epithelial cells. Fertil Steril N/A:N/A (2013). Human . Read more (PubMed: 24262600) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (MCF7 cells)
Specification MCF7 cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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投稿 Jan 07 2015

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Nos anticorps anti hMFG1 sont des anticorps monoclonaux contre la protéine totale hMFG1, et nous ne savons pas ou se situe l'épitope. Cependant, j'ai effectué une recherche d'homologie entre les formes 1 et 8 et vous trouverez en attachement les résu...

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