製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MIG
  • アプリケーション
    適用あり: ELISA, Neutralising, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Highly pure (>98%) recombinant hMIG (human Monokine induced by Interferon-Gamma)

製品の特性

  • 製品の状態
    Lyophilised:Reconstitute with 200µl of sterile water.
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    PBS, pH 7.4, no preservative, sterile filtered
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    unknown
  • 軽鎖の種類
    unknown
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab9720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use a concentration of 0.5 µg/ml.
Neutralising Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of hMIG (100.00 ng/ml), a concentration of 5.0 - 7.0 µg/ml of this antibody is required
WB Use 0.1µg for 100.2 cells.
IHC-P Use a concentration of 0.25 - 0.5 µg/ml.

ターゲット情報

  • 機能
    Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3.
  • 配列類似性
    Belongs to the intercrine alpha (chemokine CxC) family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C-X-C motif chemokine 9 antibody
    • chemokine (C-X-C motif) ligand 9 antibody
    • CMK antibody
    • crg-10 antibody
    • CXCL9 antibody
    • CXCL9_HUMAN antibody
    • gamma interferon induced monokine antibody
    • Gamma-interferon-induced monokine antibody
    • HuMIG antibody
    • MIG antibody
    • monokine induced by gamma interferon antibody
    • monokine induced by interferon gamma antibody
    • Monokine induced by interferon-gamma antibody
    • SCYB9 antibody
    • Small inducible cytokine B9 antibody
    • small inducible cytokine subfamily B member 9 antibody
    • Small-inducible cytokine B9 antibody
    see all

画像

  • ab9720 staining MIG in human renal tumor with parenchyma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.25 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

参考文献

This product has been referenced in:
  • Liu W  et al. Elevated expression of IFN-inducible CXCR3 ligands predicts poor prognosis in patients with non-metastatic clear-cell renal cell carcinoma. Oncotarget 7:13976-83 (2016). IHC . Read more (PubMed: 26910919) »
  • Chmielewski S  et al. STAT1-dependent signal integration between IFN? and TLR4 in vascular cells reflect pro-atherogenic responses in human atherosclerosis. PLoS One 9:e113318 (2014). IHC-P ; Human . Read more (PubMed: 25478796) »

See all 3 Publications for this product

レビューと Q&A

Thank you for contacting us. The product is shipped as 100 ug of lyophilized antibody. If reconstituted in 200 ul of water, the concentration will be 0.5 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if...

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Thank you for contacting us. We do not currently have any feedback from customers or lab testing regarding whether it would be possible to conjugate either of these antibodies to gold, and we therefore do not have any recommendations for a pH or conce...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil (periphery))
Specification
Tonsil (periphery)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Dr. R Berahovich

Verified customer

投稿 Dec 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal carcinoma)
Specification
Colorectal carcinoma
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 10 2011

Thank you for contacting us for technical support regarding ab9720. This antibody has not been tested on mouse tissue therefore we do not know what sort of banding pattern it gives with MIG of this species I'm afraid. There is 73% homology between the ...

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Unfortunately, this antibody has not been tested for IHC in frozen sections, so we can't guarantee that it will work.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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