製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to MIF
  • 特異性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Cow, Dog, Pig, Cynomolgus monkey, Macaque monkey
  • 免疫原

    Synthetic peptide: PMFIVNTNV PRASVPDGFL SELTQQLAQA TG conjugated to KLH, corresponding to amino acids 2-32 of Human MIF

  • ポジティブ・コントロール
    • Supernatants or lysates of endotoxin-stimulated human peripheral blood mononuclear cells (PBMC).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 精製度
    IgY fraction
  • 特記事項(精製)
    This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgY
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab34644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000 - 1/2000.
WB 1/500 - 1/1000. Detects a band of approximately 12.3 kDa (predicted molecular weight: 14 kDa).

ターゲット情報

  • 機能
    Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
  • 関連疾患
    Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
  • 配列類似性
    Belongs to the MIF family.
  • 細胞内局在
    Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GIF antibody
    • GLIF antibody
    • Glycosylation inhibiting factor antibody
    • Glycosylation-inhibiting factor antibody
    • L-dopachrome isomerase antibody
    • L-dopachrome tautomerase antibody
    • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
    • Macrophage migration inhibitory factor antibody
    • MIF antibody
    • MIF protein antibody
    • MIF_HUMAN antibody
    • MMIF antibody
    • Phenylpyruvate tautomerase antibody
    see all

Anti-MIF antibody 画像

  • All lanes : Anti-MIF antibody (ab34644) at 1/500 dilution

    Lane 1 : Control
    Lane 2 : 100 ng of recombinant MIF present in a bacterial lysate

    Secondary
    Lane 2 : Goat anti chicken at 1/5000 dilution


    Predicted band size : 14 kDa
    Observed band size : 12.3 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-MIF antibody (ab34644) 使用論文

ab34644 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab34644.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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