製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to MIF
  • 特異性
    Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Based on direct ELISA, shows no crossreactivity with most cytokines
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    E. coli-derived recombinant full length protein (Human).

  • 特記事項
    = 10ng/mg Endotoxin.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified by immunoaffinity chromatography.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: Use at a concentration of 0.5 - 1 µg/ml. Detection limit 0.15ng/well.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 13.8 kDa. Detection limit 5ng/lane.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
    • 関連疾患
      Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    • 配列類似性
      Belongs to the MIF family.
    • 細胞内局在
      Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GIF antibody
      • GLIF antibody
      • Glycosylation inhibiting factor antibody
      • Glycosylation-inhibiting factor antibody
      • L-dopachrome isomerase antibody
      • L-dopachrome tautomerase antibody
      • Macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
      • Macrophage migration inhibitory factor antibody
      • MIF antibody
      • MIF protein antibody
      • MIF_HUMAN antibody
      • MMIF antibody
      • Phenylpyruvate tautomerase antibody
      see all

    プロトコール

    Anti-MIF antibody (ab14574) 使用論文

    ab14574 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. I have found reference to the use of LPS stimulated RAW cell lysate, Neuro2A (mouse neuroblastoma cell line) lysate or a Human Thymus lysate for use as a positive control for antibodies against MIF. Unfortunately we do...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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