製品の概要

  • 製品名Anti-MIF antibody [2Ar3]
    MIF 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2Ar3] to MIF
  • 特異性Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Based on direct ELISA, shows no crossreactivity with most cytokines.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    E. coli-derived recombinant full length protein (Human).

  • 特記事項= 10ng/mg Endotoxin. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名2Ar3
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: Use at a concentration of 4 ug/ml for direct ELISA and 2 ug/ml for capture in sandwich ELISA.

    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 13.8 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.
    • 関連疾患Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    • 配列類似性Belongs to the MIF family.
    • 細胞内局在Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens.
    • Information by UniProt
    • 参照データベース
    • 別名
      • acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody
      • GIF antibody
      • GLIF antibody
      • Glycosylation inhibiting factor antibody
      • Glycosylation-inhibiting factor antibody
      • L-dopachrome isomerase antibody
      • L-dopachrome tautomerase antibody
      • Macrophage migration inhibitory factor antibody
      • MIF antibody
      • MIF protein antibody
      • MIF_HUMAN antibody
      • MMIF antibody
      • Phenylpyruvate tautomerase antibody
      see all

    Anti-MIF antibody [2Ar3] (ab14575) 使用論文

    ab14575 has not yet been referenced specifically in any publications.

    Product Wall

    The monoclonal antibody ab14575 was raised using human MIF, recombinant protein expressed in E. coli. The epitope has not been determined. For a sandwich ELISA, I recommend ab14575 for capture and goat polyclonal ab14574 for detection, followed by ...

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    Thank you for your enquiry. I have found reference to the use of LPS stimulated RAW cell lysate, Neuro2A (mouse neuroblastoma cell line) lysate or a Human Thymus lysate for use as a positive control for antibodies against MIF. Unfortunately we do...

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    Thank you for your email. If you are interested in ordering multiple vials of the same antibody, I can offer you a discount. However, if you are interested in ordering one each of the 5 antibodies that you listed, I cannot offer you a discount. Shippin...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"