製品の概要

  • 製品名Anti-MIER1 antibody
  • 製品の詳細
    Rabbit polyclonal to MIER1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide: KDASRRTGDE KGVEAIPEGS HIKDNEQALY ELVKCNFDTE EALRRLRFNV, corresponding to internal sequence amino acids 253-302 of Human MIER1

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab26254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 50 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/312500.

ターゲット情報

  • 関連性MIER1 (previously called er1) was first isolated from Xenopus laevis embryonic cells as a novel fibroblast growth factor. Human MIER1 (hMIER1) was shown to encode a nuclear protein with an N terminal acidic transcription activation domain. The human orthologue of MIER1 displays 91% similarity to the Xenopus sequence at the amino acid level and was shown to be upregulated in breast carcinoma cell lines and tumors. Alternative splicing at the 3' end of hMIER1 produces two major isoforms, hMIER1 and hMIER1ß, which contain distinct C terminal domains. The association of hMIER1 with Sp1 represents a novel mechanism for the negative regulation of Sp1 target promoters. Results demonstrate that alternate use of a facultative intron regulates the subcellular localization of hMIER1 proteins and this may have important implications for hMIER1 function in the regulation of transcription. Repressor activity is due to interaction and recruitment of a trichostatin A sensitive histone deacetylase 1 (HDAC1).
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • ER1 antibody
    • Mesoderm induction early response 1 antibody
    • Mesoderm induction early response 1 homolog (Xenopus laevis) antibody
    • MI-ER1 antibody

Anti-MIER1 antibody 画像

Anti-MIER1 antibody (ab26254) 使用論文

ab26254 has not yet been referenced specifically in any publications.

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