製品の概要

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 16 kDa).
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Has heparin binding activity, and growth promoting activity. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development.
  • 配列類似性
    Belongs to the pleiotrophin family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amphiregulin associated protein antibody
    • Amphiregulin-associated protein antibody
    • ARAP antibody
    • FLJ27379 antibody
    • Mdk antibody
    • Midgestation and kidney protein antibody
    • Midkine antibody
    • MK 1 antibody
    • MK antibody
    • MK_HUMAN antibody
    • MK1 antibody
    • NEGF 2 antibody
    • NEGF2 antibody
    • Neurite growth promoting factor 2 antibody
    • Neurite outgrowth promoting protein antibody
    • Neurite outgrowth-promoting factor 2 antibody
    • Neurite outgrowth-promoting protein antibody
    • Retinoic acid inducible factor antibody
    see all

Anti-Midkine antibody 画像

  • Anti-Midkine antibody (ab36038) at 1 µg/ml + Recombinant Midkine protein with tag at 0.1 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 16 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
    The observed molecular weight of 42 kDa is the combined molecular weight of the full-length recombinant Midkine protein (~140 amino-acids) and the tag.
  • IHC-FoFr image of Midkine (ab36038) staining on Rat Spinal cord sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the cords were cryoprotected in sucrose 30% overnight. Tissues were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique. Image B is a high power magnification of Image A.

    See Abreview

  • ICC/IF image of ab36038 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36038, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Midkine antibody (ab36038) 使用論文

This product has been referenced in:
  • He D  et al. Ethanol activates midkine and anaplastic lymphoma kinase signaling in neuroblastoma cells and in the brain. J Neurochem 135:508-21 (2015). Read more (PubMed: 26206265) »
  • Florea V  et al. c-Myc Is Essential to Prevent Endothelial Pro-Inflammatory Senescent Phenotype. PLoS One 8:e73146 (2013). Read more (PubMed: 24039874) »

See all 2 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Loading amount
30 µg
Specification
Kidney
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Dec 30 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Specification
Spinal cord
Fixative
Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 06 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録