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Our Abpromise guarantee covers the use of ab14749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 81 kDa.Can be blocked with Human MID2 peptide (ab23250).
Approx 80-85kDa band observed in Mouse and Rat Heart, and Rat Kidney lysates (calculated MW of 77.8kDa according to Mouse NP_035975.1 and 83.1kDa according to Rat NP_001178818.1).
ab14749 at 5ug/ml staining MID2 in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in steam with citrate buffer (pH 6.0). The AP-staining procedure was used for detection.
ab14749 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"