The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 78 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
組織特異性Low level in fetal kidney and lung, and in adult prostate, ovary and small intestine.
パスウェイProtein modification; protein ubiquitination.
配列類似性Belongs to the TRIM/RBCC family. Contains 2 B box-type zinc fingers. Contains 1 B30.2/SPRY domain. Contains 1 COS domain. Contains 1 fibronectin type-III domain. Contains 1 RING-type zinc finger.
ドメインThe tripartite motif (RBCC; RING- and B box-type zinc fingers and coiled coil domains) mediates dimerization. Associates with microtubules in a manner that is dependent on the C-terminal B30.2 domain.
翻訳後修飾Phosphorylated on serine and threonine residues.