製品の概要

  • 製品名Anti-MHF2 antibody
    MHF2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MHF2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque Monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human MHF2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Liver tissue lysate as well as the following human whole cell lysates: HEK293; U2OS; HepG2.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 9 kDa).

ターゲット情報

  • 機能DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation.
  • 配列類似性Belongs to the CENPX family.
  • 細胞内局在Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CENP X antibody
    • CENP-X antibody
    • CENPX antibody
    • CENPX_HUMAN antibody
    • Centromere protein X antibody
    • FAAP10 antibody
    • FANCM interacting histone fold protein 2 antibody
    • FANCM-interacting histone fold protein 2 antibody
    • Fanconi anemia associated polypeptide of 10 kDa antibody
    • Fanconi anemia-associated polypeptide of 10 kDa antibody
    • MGC14480 antibody
    • Retinoic acid inducible gene D9 protein homolog antibody
    • Retinoic acid-inducible gene D9 protein homolog antibody
    • Stimulated by retinoic acid gene 13 protein homolog antibody
    • STRA13 antibody
    see all

Anti-MHF2 antibody 画像

  • All lanes : Anti-MHF2 antibody (ab104236) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 9 kDa
    Observed band size : 11 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 160 kDa,46 kDa,51 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-MHF2 antibody (ab104236) 使用論文

ab104236 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab104236.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"