製品の概要

  • 製品名
    Anti-MHF2 antibody
  • 製品の詳細
    Rabbit polyclonal to MHF2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human MHF2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Liver tissue lysate as well as the following human whole cell lysates: HEK293; U2OS; HepG2.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab104236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 11 kDa (predicted molecular weight: 9 kDa).

ターゲット情報

  • 機能
    DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Recruited to forks stalled by DNA interstrand cross-links, and required for cellular resistance to such lesions. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation.
  • 配列類似性
    Belongs to the CENPX family.
  • 細胞内局在
    Nucleus. Chromosome > centromere. Chromosome > centromere > kinetochore. Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CENP X antibody
    • CENP-X antibody
    • CENPX antibody
    • CENPX_HUMAN antibody
    • Centromere protein X antibody
    • FAAP10 antibody
    • FANCM interacting histone fold protein 2 antibody
    • FANCM-interacting histone fold protein 2 antibody
    • Fanconi anemia associated polypeptide of 10 kDa antibody
    • Fanconi anemia-associated polypeptide of 10 kDa antibody
    • MGC14480 antibody
    • Retinoic acid inducible gene D9 protein homolog antibody
    • Retinoic acid-inducible gene D9 protein homolog antibody
    • Stimulated by retinoic acid gene 13 protein homolog antibody
    • STRA13 antibody
    see all

画像

  • All lanes : Anti-MHF2 antibody (ab104236) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 9 kDa
    Observed band size: 11 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 160 kDa, 46 kDa, 51 kDa, 58 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 2 minutes


    ab104236 detects a band at 11 kDa. While this differs to its predicted molecular weight of 9 kDa, the expression profile observed is consistent with what has been described in the literature PMID:20347429. This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab104236 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

プロトコール

参考文献

ab104236 has not yet been referenced specifically in any publications.

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