製品の概要

  • 製品名
    Anti-MHC Qa1b antibody [6A8.6F10.1A6]
  • 製品の詳細
    Mouse monoclonal [6A8.6F10.1A6] to MHC Qa1b
  • アプリケーション
    適用あり: WB, IP, Neutralising, Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide (Mouse) MHC Qa1b

  • ポジティブ・コントロール
    • ConA-stimulated Balb/c mouse splenocytes.
  • 特記事項


    ab72487 can be used for flow cytometric assay to detect Qa1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa1 restricted antigen presentation and other functional study where Qa1b associated immuno-regulation may work.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 一次抗体 備考
    ab72487 can be used for flow cytometric assay to detect Qa1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa1 restricted antigen presentation and other functional study where Qa1b associated immuno-regulation may work.
  • ポリ/モノ
    モノクローナル
  • クローン名
    6A8.6F10.1A6
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 41 kDa.
IP Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration.
Flow Cyt Use 0.1-0.5µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性
    Qa1b is a murine nonclassical MHC class I molecule, encoded by the T23 gene in the T region. It functions in both innate and adaptive immune responses, regulating NK cell activation and serving as a restriction element for a subpopulation of CD8+ T cells. It is expressed in a wide range of tissues similar to MHC class Ia molecules. However, Qa1b is relatively nonpolymorphic and is expressed at lower levels than MHC class Ia molecules. In contrast to classical MHC class I molecules, Qa1b predominantly binds a single nonameric peptide, Qa1b determinant modifier (Qdm), derived from the signal sequence of H-2D/L molecules. The Qa-1/Qdm complex serves as a ligand for CD94/NKG2 natural killer (NK) receptor(s) expressed on a major fraction of NK cells and CD8+ immuno-suppressive regulatory T cells. Qa-1b, expressed on immuno-regulatory B cells and other antigen presenting cells, serves for antigen-presentation to immuno-regulatory CD8+ T. Moreover, Qa-1b serves an MHC restrictive function because, unlike conventional MHC molecules, it transiently expresses on activated CD4+ T cells. Recently it has also been shown that Qa-1b is ubiquitously expressed in the immune-privileged anterior chamber of the eye. Additionally, it has been suggested that TCR specific, Qa-1b-restricted regulatory CD8+ T cells selectively down regulate antigen-activated T cells expressing TCRs of certain affinities. These regulatory CD8+ T cells may control the peripheral TCR repertoire during the course of immune responses to both self and foreign antigens. HLA-E is the functional ortholog of Qa-1b in humans.
  • 細胞内局在
    Membrane; Single-pass type I membrane protein.
  • 参照データベース
  • 別名
    • H2 class I histocompatibility antigen D37 alpha chain antibody
    • H2 Q1 antibody
    • H2 T23 antibody
    • Histocompatibility 2 T region locus 23 antibody
    • MHC class I antigen Qa1 antibody
    • MHC classIb Qa1 antibody
    • OTTMUSP00000015895 antibody
    • Qa1 antibody
    see all

画像

  • Flow cytometric analysis of MHC Qa1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) ab72487 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/106 cells. A shift is seen in only a small percentage of cells.
  • Flow cytometric analysis of MHC Qa1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) ab72487 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/106 cells. A shift is seen in the entire population of cells.

参考文献

This product has been referenced in:
  • Gan Y  et al. Ischemic neurons recruit natural killer cells that accelerate brain infarction. Proc Natl Acad Sci U S A 111:2704-9 (2014). ICC/IF ; Mouse . Read more (PubMed: 24550298) »

See 1 Publication for this product

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