The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 0.1-0.5µg for 106 cells. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. Also cross-reacts with mouse strains (MHC haplotypes b and s ) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9 (1). Ia antigens are recognized on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes (1,3,4). Thus, is particularly useful for distinguishing Ia positive cells from different rat strains
and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
Type I membrane protein.
major histocompatibility complex antibody
major histocompatibility complex class II antibody
Anti-MHC class II RT Ib antibody [MRC OX-6] (Biotin) 画像
Flow Cytometry - MHC class II RT Ib antibody [MRC OX-6] (Biotin) (ab112108)
ab112108 used at 0.1µg/106 cells staining MHC class II RT Ib in Rat spleen cells (Fischer strain).
Percentage of cells stained above control (Biotin Mouse IgG1,k) is 39.0%.