Anti-MHC class II RT Ib 抗体 [F17-23-2] (ab33745)

製品の概要

  • 製品名
    Anti-MHC class II RT Ib antibody [F17-23-2]
  • 製品の詳細
    Mouse monoclonal [F17-23-2] to MHC class II RT Ib
  • 特異性
    Recognizes the rat RT1B MHC class II antigen, reacting with haplotypes a, l and n expressed on rat strains including DA, LEW, and BN.
  • アプリケーション
    適用あり: Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Partially purified MHC antigens (Rat)

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: 5-10% Foetal calf serum, PBS, 0.2M Tris HCl. pH 7.4
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    F17-23-2
  • ミエローマ
    NS1
  • アイソタイプ
    IgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IHC-Fr
  • 追加情報
    Flow Cyt: Use 50µl of 1/5 diluted antibody sample for 106 cells in 100µl.
    IHC-Fr: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      Recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. Also cross-reacts with mouse strains (MHC haplotypes b and s ) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9 (1). Ia antigens are recognized on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes (1,3,4). Thus, is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
    • 細胞内局在
      Type I membrane protein.
    • 別名
      • major histocompatibility complex antibody
      • major histocompatibility complex class II antibody

    参考文献

    This product has been referenced in:
    • Mirenda V  et al. Modified dendritic cells coexpressing self and allogeneic major histocompatability complex molecules: an efficient way to induce indirect pathway regulation. J Am Soc Nephrol 15:987-97 (2004). Read more (PubMed: 15034101) »

    See 1 Publication for this product

    レビューと Q&A

    Merci de votre appel de toutà l'heure.

    Voici les résultats du blast promises. Comme discuté au téléphone, nous avons aussi des anticorps contre le RT1 class Ib de rat directement, comme par exemple le ab3374...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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