The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: Use 50µl of 1/5 diluted antibody sample for 106 cells in 100µl.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Recognizes a polymorphic determinant on rat Ia present on Lewis, Wistar, and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN, DA, or PVG/c strains. Also cross-reacts with mouse strains (MHC haplotypes b and s ) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9 (1). Ia antigens are recognized on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes (1,3,4). Thus, is particularly useful for distinguishing Ia positive cells from different rat strains
and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.
Type I membrane protein.
major histocompatibility complex antibody
major histocompatibility complex class II antibody
Mirenda V et al. Modified dendritic cells coexpressing self and allogeneic major histocompatability complex molecules: an efficient way to induce indirect pathway regulation. J Am Soc Nephrol15:987-97 (2004).
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