Anti-MHC class II I E kappa 抗体 [17-3-3.4] (FITC) (ab25679)

製品の概要

  • 製品名Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC)
    MHC class II I E kappa 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [17-3-3.4] to MHC class II I E kappa (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性This antibody reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype; reactivity with H-2d and H-2p haplotypes has not been observed. [Cells from mice of the H-2b, H-2f, H-2g7, H-2q, and H-2s haplotypes do not express I-E antigen.]
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation (Mouse) -C3H mouse skin graft and splenocytes.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名17-3-3.4
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報Flow Cyt: Use 1µg for 106 cells.
    Complement-mediated cytotoxicity: Use at an assay dependent dilution


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
    • 細胞内局在Type I membrane protein
    • 別名
      • H2 Ea antibody
      • H2-Ea antibody

    Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) 画像

    • AKR spleen cells were double-stained with ab25679 (at 1µg/106 cells) and Rat Anti-Mouse CD3ε-R-PE.  Lymphocytes were gated using and analyzed by flow cytometry.

    Anti-MHC class II I E kappa antibody [17-3-3.4] (FITC) (ab25679) 使用論文

    This product has been referenced in:
    • Ozato K  et al. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol 124:533-40 (1980). Read more (PubMed: 7188699) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"