Mouse monoclonal [17-3-3.4] to MHC class II I E kappa (Biotin)
ab25226 reacts with the I-Ek class II alloantigen on cells from mice of the H-2r haplotype; reactivity with H-2d and H-2p haplotypes has not been observed. Cells from mice of the H-2b, H-2f, H-2g7, H-2q,
and H-2s haplotypes do not express I-E antigen. can be used for complement-mediated cytotoxicity.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use 1µg for 106 cells. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.