Anti-MHC class II I E kappa 抗体 [14-4-4S] (Phycoerythrin) (ab25585)

製品の概要

  • 製品名Anti-MHC class II I E kappa antibody [14-4-4S] (Phycoerythrin)
    MHC class II I E kappa 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14-4-4S] to MHC class II I E kappa (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性This antibody is specific to an epitope on mouse I-E? MHC class II alloantigen. The antibody reacts with the I-E? class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. The antibody has also been reported to crossreact with the rat class II alloantigen RT1D.
  • アプリケーション適用あり: Flow Cyt, IP, IHC-Fr, Blockingmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation: C3H mouse skin graft and splenocytes.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • クローン名14-4-4S
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25585 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IP
IHC-Fr
Blocking
  • 追加情報Flow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr(cetone fixed): Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    BL: Use at an assay dependent dilution for in vitro blocking of antigen presentation.
    Complement dependent cytotoxicity: Use at an assay dependent dilution.
    In vitro induction of antigen specific B cell differentiation: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性A major histocompatibility complex class II receptor. These display processed antigens from virally infected or transformed cells. Class II positive cells ('antigen presenting cells') can take up antigens from outside by endocytosis, degrade them into small peptides, and re export the peptides (now bound to MHC class II protein) to the cell surface. These peptide MHC class II complexes can then be recognized by specific CD4+ lymphocytes.
    • 細胞内局在Type I membrane protein
    • 別名
      • H2 Ea antibody
      • H2-Ea antibody

    Anti-MHC class II I E kappa antibody [14-4-4S] (Phycoerythrin) (ab25585) 使用論文

    This product has been referenced in:
    • Farr A  et al. Differential expression of CLIP:MHC class II and conventional endogenous peptide:MHC class II complexes by thymic epithelial cells and peripheral antigen-presenting cells. Eur J Immunol 26:3185-93 (1996). Read more (PubMed: 8977321) »
    • Roy M  et al. Studies on the interdependence of gp39 and B7 expression and function during antigen-specific immune responses. Eur J Immunol 25:596-603 (1995). Read more (PubMed: 7533092) »

    See all 5 Publications for this product

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    There are currently no Abreviews or Questions for ab25585.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"