ab63567 recognises a polymorphic determinant of rat Ia antigen (RT1Bu) present on Lewis, Wistar and AO strain rats but not BN, DA or PVG/c strains; reacts with mouse strains of MHC haplotypes b and s, and analysis of recombinant mouse strains showed that the determinants mapped to the I-A region; routinely tested in flow cytometry on Lewis rat splenocytes.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/100. Use 10µl of the suggested working dilution to label 106) cells in 100µl.
IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. Does not require antigen retrieval methods.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Belongs to the MHC class II family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Ubiquitinated in immature dendritic cells leading to down-regulation of MHC class II.
McMaster WR & Williams AF Monoclonal antibodies to Ia antigens from rat thymus: cross reactions with mouse and human and use in purification of rat Ia glycoproteins. Immunol Rev47:117-37 (1979).
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