Anti-MHC Class II 抗体 [M5/114.15.2] (Phycoerythrin) (ab93560)


  • 製品名Anti-MHC Class II antibody [M5/114.15.2] (Phycoerythrin)
    MHC Class II 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [M5/114.15.2] to MHC Class II (Phycoerythrin)
  • 標識Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion encoded glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u but not from mice carrying the H-2s or H-2f haplotypes. The M5/114 mnonclonal antibody is reported to inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, or H-2s haplotypes.
  • アプリケーション適用あり: Flow Cytmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Tissue/ cell preparation (Mouse): Activated C57BL/6 Mouse spleen cells.

  • ポジティブ・コントロール
    • C57Bl/6 Mouse splenocytes


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS, 150mM Sodium chloride, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名M5/114.15.2
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野


Our Abpromise guarantee covers the use of ab93560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
  • 追加情報Flow Cyt: Use at a concentration of 0.0075 - 0.02 µg/test in a 100 µl volume.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
    • 配列類似性Belongs to the MHC class II family.
      Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
    • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • D6S221E antibody
      • DMA antibody
      • DMB antibody
      • DP beta 1 antibody
      • DP beta 1 chain antibody
      • DP(W4) beta chain antibody
      • DPB 1 antibody
      • DPB1 antibody
      • DPB1_HUMAN antibody
      • DRB antibody
      • H2Ea antibody
      • HLA class II histocompatibility antigen antibody
      • HLA class II histocompatibility antigen DM beta chain antibody
      • HLA DMB antibody
      • HLA DPB1 antibody
      • HLA-DP antibody
      • HLA-DP1B antibody
      • HLA-DPB antibody
      • HLA-DPB1 antibody
      • HLADM antibody
      • HLADP1B antibody
      • Major histocompatibility complex class II antibody
      • Major histocompatibility complex class II DP beta 1 antibody
      • MHC class II antigen DMB antibody
      • MHC class II antigen DPB1 antibody
      • MHC DPB1 antibody
      • RING6 antibody
      • RING7 antibody
      see all

    Anti-MHC Class II antibody [M5/114.15.2] (Phycoerythrin) 画像

    • ab93560, at 0.0075 µg, staining MHC Class II in C57Bl/6 splenocytes by Flow Cytometry. Total viable cells were used for analysis.

    Anti-MHC Class II antibody [M5/114.15.2] (Phycoerythrin) (ab93560) 使用論文

    This product has been referenced in:
    • Gueirard P  et al. Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract. Infect Immun 71:4137-43 (2003). Read more (PubMed: 12819105) »
    • Bagavant H  et al. Induction and immunohistology of autoimmune ovarian disease in cynomolgus macaques (Macaca fascicularis). Am J Pathol 160:141-9 (2002). Read more (PubMed: 11786408) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (Spleen cell)
    Permeabilization No
    Gating Strategy CD45, CD11c, MHCII
    Specification Spleen cell
    Preparation Cell harvesting/tissue preparation method: Spleen smushing through 70um filter, then RBC lysis
    Sample buffer: PBS + 2% FBS
    Fixation Formaldehyde

    Ms. Cheryl Sandoval

    Verified customer

    投稿 Jun 28 2016

    According to the literature Balb/c mice have the following haplotype: I-Ad and I-Ed and H-2d . (

    ab93560 recognizes I-Ab, I-Ad, I-Aq,...

    Read More