Anti-MHC Class II 抗体 [ER-TR3] (Biotin) (ab15631)


  • 製品名Anti-MHC Class II antibody [ER-TR3] (Biotin)
    MHC Class II 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [ER-TR3] to MHC Class II (Biotin)
  • 標識Biotin
  • 特異性The antigen is found on B-cells, interdigitating cells and macrophages in peripheral lymphoid organs but is absent from T-cells. It is also expressed as a fine reticular pattern on stromal thymic cells of the cortex and as a confluent pattern on stromal thymic cells of the medulla.
  • アプリケーション適用あり: IHC-Fr, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    Full length native protein (purified) (Mouse).

  • ポジティブ・コントロール
    • Mouse dendritic cells, B-cells and macrophages.



  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal
    Constituents: 10mg/ml BSA, PBS, pH 7.2
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified IgG from culture supernatant
  • ポリ/モノモノクローナル
  • クローン名ER-TR3
  • アイソタイプIgG2b
  • 研究分野


Our Abpromise guarantee covers the use of ab15631 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100.
ICC/IF Use at an assay dependent concentration. PubMed: 24465914
Flow Cyt Use at an assay dependent concentration. ab18542-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • 機能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 配列類似性Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D6S221E antibody
    • DMA antibody
    • DMB antibody
    • DP beta 1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB 1 antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DRB antibody
    • H2Ea antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DM beta chain antibody
    • HLA DMB antibody
    • HLA DPB1 antibody
    • HLA-DP antibody
    • HLA-DP1B antibody
    • HLA-DPB antibody
    • HLA-DPB1 antibody
    • HLADM antibody
    • HLADP1B antibody
    • Major histocompatibility complex class II antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • MHC class II antigen DMB antibody
    • MHC class II antigen DPB1 antibody
    • MHC DPB1 antibody
    • RING6 antibody
    • RING7 antibody
    see all

Anti-MHC Class II antibody [ER-TR3] (Biotin) (ab15631) 使用論文

This product has been referenced in:
  • Danzl NM  et al. Identification of novel thymic epithelial cell subsets whose differentiation is regulated by RANKL and Traf6. PLoS One 9:e86129 (2014). ICC/IF ; Mouse . Read more (PubMed: 24465914) »

See 1 Publication for this product

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