製品の概要

  • 製品名Anti-MHC Class II antibody
    MHC Class II 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MHC Class II
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 76-106 of Human MHC Class II (NP_002112.3).

  • ポジティブ・コントロール
    • 293 cell lysates transiently transfected with the MHC class II gene. IF/ICC: A431 cell line

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab116378 is purified through a protein A column, followed by peptide affinity purification.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab116378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 29 kDa.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
  • 配列類似性Belongs to the MHC class II family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
  • 細胞内局在Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D6S221E antibody
    • DMA antibody
    • DMB antibody
    • DP beta 1 antibody
    • DP beta 1 chain antibody
    • DP(W4) beta chain antibody
    • DPB 1 antibody
    • DPB1 antibody
    • DPB1_HUMAN antibody
    • DRB antibody
    • H2Ea antibody
    • HLA class II histocompatibility antigen antibody
    • HLA class II histocompatibility antigen DM beta chain antibody
    • HLA class II histocompatibility antigen, DP beta 1 chain antibody
    • HLA class II histocompatibility antigen, DP(W4) beta chain antibody
    • HLA DMB antibody
    • HLA DP1A antibody
    • HLA DPB1 antibody
    • HLA SB alpha chain antibody
    • HLA-A antibody
    • HLA-A histocompatibility type antibody
    • HLA-DP antibody
    • HLA-DP histocompatibility type, beta-1 subunit antibody
    • HLA-DP1B antibody
    • HLA-DPB antibody
    • HLA-DPB1 antibody
    • HLADM antibody
    • HLADP1B antibody
    • HLASB antibody
    • HLASB histocompatibility type antibody
    • Human MHC class II HLA SB alpha antibody
    • LA class II histocompatibility antigen DP alpha 1 chain antibody
    • Major histocompatibility complex class II antibody
    • Major histocompatibility complex class II DP alpha 1 antibody
    • Major histocompatibility complex class II DP beta 1 antibody
    • Major histocompatibility complex, class I, A antibody
    • MHC class II antigen DMB antibody
    • MHC class II antigen DPB1 antibody
    • MHC class II DP3 alpha antibody
    • MHC class II DPA1 antibody
    • MHC class II HLA-DP-beta-1 antibody
    • MHC DPB1 antibody
    • MHC HLA DPB1 antibody
    • PLT1 antibody
    • Primed lymphocyte test 1 antibody
    • RING6 antibody
    • RING7 antibody
    see all

Anti-MHC Class II antibody 画像

  • ICC/IF image of ab116378 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab116378, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-MHC Class II antibody (ab116378) at 1/100 dilution

    Lane 1 : 293 cell lysate, non-transfected
    Lane 2 : 293 cell lysate, transfected with MHC Class II gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 29 kDa

Anti-MHC Class II antibody (ab116378) 使用論文

This product has been referenced in:
  • Li Y & Ding J Optimized generation of survivin-specific cytotoxic T lymphocytes against lung cancer. Mol Med Rep 12:2169-74 (2015). Read more (PubMed: 25845433) »

See 1 Publication for this product

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I assume you were refering to ab107604 as the L243 antibody (http://www.abcam.com/hla-dr-antibody-l243-phycoerythrin-ab107604.html)? This antibody has not been tested in WB yet and is PE-conjugated. Please feel free to...

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I have searched our catalog and unfortunately we do not currently have any anti-MHC Class II antibodies which have been tested in hamster. Looking further at the hamster protein against the mouse, rat and ...

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