Anti-MHC class I RT Ia 抗体 [OX-18] (ab33752)
Key features and details
- Mouse monoclonal [OX-18] to MHC class I RT Ia
- Suitable for: ELISA, IP, Flow Cyt, IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
製品の概要
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製品名
Anti-MHC class I RT Ia antibody [OX-18] -
製品の詳細
Mouse monoclonal [OX-18] to MHC class I RT Ia -
由来種
Mouse -
特異性
Ab33752 recognises a monomorphic determinant of rat MHC Class I (RT1A), expressed by all rat strains. However, quantitative measurements suggest that not all of the class I molecules are recognised. -
アプリケーション
適用あり: ELISA, IP, Flow Cyt, IHC-Frmore details -
種交差性
交差種: Rat -
免疫原
Full length protein corresponding to Rat MHC class I RT Ia. Spleen cell glycoproteins.
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特記事項
This clone (OX-18) has been used in immunoaffinity purification of rat MHC class I molecules.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
This antibody was purified tissue culture supernatant. -
一次抗体 備考
This clone (OX-18) has been used in immunoaffinity purification of rat MHC class I molecules. -
ポリ/モノ
モノクローナル -
クローン名
OX-18 -
ミエローマ
P3x63-Ag8.653 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab33752の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
Use 10ul of this dilution for 10^6 cells in 100ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-Fr |
Use at an assay dependent concentration.
Acetone fixation recommended. The antigen is sensitive to fixation with paraformaldehyde. |
特記事項 |
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ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Use 10ul of this dilution for 10^6 cells in 100ul. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-Fr
Use at an assay dependent concentration. Acetone fixation recommended. The antigen is sensitive to fixation with paraformaldehyde. |
ターゲット情報
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関連性
The major histocompatibility complex (MHC) plays a central role in controlling immune responsiveness and susceptibility to a great variety of mainly autoimmune and infectious diseases. The mammalian MHC is usually divided into three regions, the class I, II, and III (also called central) gene regions, based on the genes contained therein. Class I regions encode the classical I (class Ia) and nonclassical I (class Ib) molecules. The widely expressed class Ia molecules perform the well described antigen presenting function to CD8 T lymphocytes, whereas the function of class Ib genes has largely remained unknown. -
細胞内局在
Secreted -
参照データベース
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別名
- RT1 class I gene (A 1) antibody
- RT1 class I gene antibody
- RT1 class Ia locus A antibody
see all
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab33752 は 4 報の論文で使用されています。
- Heuer A et al. hESC-derived neural progenitors prevent xenograft rejection through neonatal desensitisation. Exp Neurol 282:78-85 (2016). IHC ; Rat . PubMed: 27235932
- Wu ZP et al. Major histocompatibility complex class I-mediated inhibition of neurite outgrowth from peripheral nerves. Immunol Lett 135:118-23 (2011). PubMed: 20974178
- Smits KM et al. Preincubation of tumor cells with the anti-(MHC) class I antibody OX18 enhances tumor cell lysis by interleukin-2-activated NK cells. Transplant Proc 25:2806-7 (1993). PubMed: 8212242
- Fukumoto T et al. Mouse monoclonal antibodies against rat major histocompatibility antigens. Two Ia antigens and expression of Ia and class I antigens in rat thymus. Eur J Immunol 12:237-43 (1982). PubMed: 6178598