Anti-MHC Class I H2 Kd 抗体 [34-1-2S] (ab25334)

製品の概要

  • 製品名Anti-MHC Class I H2 Kd antibody [34-1-2S]
    MHC Class I H2 Kd 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [34-1-2S] to MHC Class I H2 Kd
  • 特異性ab25334 binds to a common determinant in the a3 domains of H-2Kd and H-2Dd in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Kb.
  • アプリケーション適用あり: Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    C3H.SW splenocytes (Mouse)

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノモノクローナル
  • クローン名34-1-2S
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IHC-Fr
  • 追加情報Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    Complement-mediated cytotoxicity: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性MHC Class I H2 Kd is involved in the presentation of foreign antigens to the immune system. The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
    • 細胞内局在Cell Membrane
    • 参照データベース
    • 別名
      • class I major histocompatibility antigen H 2Kd antibody
      • H 2 class I histocompatibility antigen K D alpha chain [Precursor] antibody
      • H 2K antibody
      • H2 K1 antibody
      • MHC class I H2 K b alpha 2 cell surface glycoprotein antibody
      • MHC class I heavy chain H2 K antibody
      • MHC H2 K transplantation antigen antibody
      • MHC K bm6 transplantation antigen antibody
      see all

    Anti-MHC Class I H2 Kd antibody [34-1-2S] (ab25334) 使用論文

    This product has been referenced in:
    • Allen H  et al. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A 83:7447-51 (1986). Read more (PubMed: 3532114) »
    • Allen H  et al. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature 309:279-81 (1984). Read more (PubMed: 6201750) »

    See all 4 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Mouse Cell (RAW264.7 cell line)
    Permeabilization No
    Specification RAW264.7 cell line
    Fixative acetone/methanol 50/50
    Username

    Dr. Julian Bursztyka

    Verified customer

    投稿 Nov 13 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample Mouse Tissue sections (Brain section)
    Specification Brain section
    Permeabilization Yes - 0.1M PBS with 3% TritonX100
    Fixative Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Nov 05 2014



    I am very pleased to hear you would like to accept our offer and test ab25334 in IHC-P. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"