Anti-MHC Class I H2 Kd 抗体 [34-1-2S] (ab25334)

製品の概要

  • 製品名
    Anti-MHC Class I H2 Kd antibody [34-1-2S]
    MHC Class I H2 Kd 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [34-1-2S] to MHC Class I H2 Kd
  • 特異性
    ab25334 binds to a common determinant in the a3 domains of H-2Kd and H-2Dd in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Kb.
  • アプリケーション
    適用あり: Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    C3H.SW splenocytes (Mouse)

  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • 精製度
    IgG fraction
  • ポリ/モノ
    モノクローナル
  • クローン名
    34-1-2S
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
IHC-Fr
  • 追加情報
    Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    Complement-mediated cytotoxicity: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      MHC Class I H2 Kd is involved in the presentation of foreign antigens to the immune system. The “classical” MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.
    • 細胞内局在
      Cell Membrane
    • 参照データベース
    • 別名
      • class I major histocompatibility antigen H 2Kd antibody
      • H 2 class I histocompatibility antigen K D alpha chain [Precursor] antibody
      • H 2K antibody
      • H2 K1 antibody
      • MHC class I H2 K b alpha 2 cell surface glycoprotein antibody
      • MHC class I heavy chain H2 K antibody
      • MHC H2 K transplantation antigen antibody
      • MHC K bm6 transplantation antigen antibody
      see all

    参考文献

    This product has been referenced in:
    • Allen H  et al. Beta 2-microglobulin is not required for cell surface expression of the murine class I histocompatibility antigen H-2Db or of a truncated H-2Db. Proc Natl Acad Sci U S A 83:7447-51 (1986). Read more (PubMed: 3532114) »
    • Allen H  et al. Domain interactions of H-2 class I antigens alter cytotoxic T-cell recognition sites. Nature 309:279-81 (1984). Read more (PubMed: 6201750) »

    See all 4 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RAW264.7 cell line)
    Permeabilization
    No
    Specification
    RAW264.7 cell line
    Fixative
    acetone/methanol 50/50
    Username

    Dr. Julian Bursztyka

    Verified customer

    投稿 Nov 13 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (Brain section)
    Specification
    Brain section
    Permeabilization
    Yes - 0.1M PBS with 3% TritonX100
    Fixative
    Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    投稿 Nov 05 2014



    I am very pleased to hear you would like to accept our offer and test ab25334 in IHC-P. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview for IHC-P and include this code...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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