Anti-MHC Class I H2 Kb 抗体 (ab93364)

製品の概要

  • 製品名
    Anti-MHC Class I H2 Kb antibody
  • 製品の詳細
    Rabbit polyclonal to MHC Class I H2 Kb
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Recombinant fragment
  • 免疫原

    Synthetic peptide corresponding an internal sequence of Mouse MHC Class I H2 Kb.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purity >90%
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab93364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA 1/2000 - 1/5000.

ターゲット情報

  • 関連性
    H2-Kb belongs to the family of the mouse Major Histocompatibility Complex class I receptors. These display processed antigens from the endocytosed bacteria. MHC class I proteins can form complexes with processed antigen peptides only if the antigen is synthesized in the same cell. As a consequence, T lymphocytes can only bind to class-I-positive cells that are synthesizing the antigen (e.g. virus-infected cells).
  • 細胞内局在
    Cell Membrane
  • 参照データベース
  • 別名
    • H2 class I histocompatibility antigen antibody
    • H2K antibody
    • H2K(B) antibody
    • H2K1 antibody
    • KB alpha chain antibody
    see all

Anti-MHC Class I H2 Kb antibody 画像

  • All lanes : Anti-MHC Class I H2 Kb antibody (ab93364) at 1 µg/ml

    Lane 1 : Whole cell lysate prepared from murine insulinoma cells MIN6 untreated.
    Lane 2 : Whole cell lysate prepared from murine insulinoma cells MIN6 treated with 60U/ml interferon-gamma for 72 hours.

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP conjugated goat anti-rabbit (H+L) polyclonal at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

プロトコール

Anti-MHC Class I H2 Kb antibody (ab93364) 使用論文

ab93364 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Colon Cancer Cell Line- CT26)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
30 µg
Specification
Mouse Colon Cancer Cell Line- CT26
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Aug 19 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.1M PBS with 3% TritonX100
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

投稿 Nov 05 2014

Application
Western blot
Loading amount
1.2e+007 cells
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Tissue lysate - whole (spleenocytes)
Specification
spleenocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jan 17 2014

we have issued a free of charge replacement.

Thank you for contacting us.

Your credit note ID is 23807.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used i...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application us...

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Application
Western blot
Sample
Mouse Cell lysate - whole cell (murine insulinoma cells MIN6)
Loading amount
25 µg
Specification
murine insulinoma cells MIN6
Treatment
60U/ml interferon-gamma for 72hours
Gel Running Conditions
Reduced Denaturing (10% Resolving gel, 5% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Apr 19 2012

DISCOUNT CODE: xx Ablaufdatum: 20. Juli 2012

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab93364 im Western blot gegen das endogene Mausprotein zu testen, annehmen möchten. Wie am Telefon besprochen, können Sie den Guts...

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Thank you for contacting us. We have only tested this antibody with recombinant fragment which we used as immunogen. We have not tested it using endogenous protein or lysates. This is the reason why the mouse species is predicted not tested. Ab...

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Thank you for contacting us. I can confirm that using this MHC Class I H2 Kb antibody ab93364 a lower band can be expected at around 40 kDa which is due to the non-glycosylated form of the protein. The upper band is the glycosylated mature prot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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