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Tissue/ cell preparation: C57BL/6 x DBA/2 F1 hybrid mouse spenocytes.
This antibody has been reported to block the recognition of H2Dd by Ly49A+, Ly49C+, and Ly49G2+ natural killer cells. The antibody has also been shown to be useful in studies of complement dependent cytotoxicity, and has been used for immunoaffinity chromatography.
Our Abpromise guarantee covers the use of ab25590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells. ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|Blocking||Use at an assay dependent concentration.
Blocking of antigen recognition.
|IP||Use at an assay dependent concentration.|
ab25590 has not yet been referenced specifically in any publications.