Anti-MHC class I 抗体 [OX-18] (FITC) (ab22367)

製品の概要

  • 製品名Anti-MHC class I antibody [OX-18] (FITC)
    MHC class I 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [OX-18] to MHC class I (FITC)
  • 標識FITC. Ex: 493nm, Em: 528nm
  • 特異性ab22367 recognises a monomorphic determinant of rat MHC Class I (RT-1A), however quantitative measurements suggest that not all of the Class I molecules are recognised.
  • アプリケーション適用あり: IHC-P, IHC-R, ELISA, Flow Cyt, IHC-Fr, ICC/IF, Othermore details
  • 種交差性
    交差種: Rat
  • 免疫原

    Full length protein: Rat spleen cell glycoprotein

  • ポジティブ・コントロール
    • Flow cytometry: rat splenocytes

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名OX-18
  • ミエローマP3x63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-R Use at an assay dependent concentration. Note for IHC: Acetone fixation recommended. The antigen is sensitive to fixation with paraformaldehyde.
AP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells.

Use 10µl of suggested working dilution (neat - 1/10) for 106 cells in 100µl.

ab91356 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/20.
Other Use at an assay dependent concentration.

ターゲット情報

  • 関連性MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • 細胞内局在Cell Membrane; Type I membrane protein.
  • 別名
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

Anti-MHC class I antibody [OX-18] (FITC) (ab22367) 使用論文

This product has been referenced in:
  • Sjöqvist S  et al. Experimental orthotopic transplantation of a tissue-engineered oesophagus in rats. Nat Commun 5:3562 (2014). IHC-Fr ; Rat . Read more (PubMed: 24736316) »
  • Akeno N  et al. IFN-{alpha} Mediates the Development of Autoimmunity both by Direct Tissue Toxicity and through Immune Cell Recruitment Mechanisms. J Immunol 186:4693-706 (2011). Flow Cyt ; Rat . Read more (PubMed: 21402899) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Pancreatic acinar cells)
Specification Pancreatic acinar cells
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Dec 08 2011

Thank you for your patience in this matter. The originator of ab22367 (MHC class I antibody [OX-18] (FITC)) has provided the following information: OX-18 is listed as being against a monomorphic determinant because it reacted with all rat str...

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Thank you for your enquiry. The antibody has not been tested on rat testis cells therefore we cannot recommend a specific amount of cells to use for this tissue. We know that the antibody worked well on rat splenocytes when 10ul of the suggested wor...

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Thank you for your enquiry. ab22367 recognizes rat MHC 1 molecules, which is generally known to be expressed on virtually all somatic, nucleated cells. We could not recognise "dlsatic fibers", so we are not really able to comment on whether we would...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"