Anti-MHC class I 抗体 [ERMP42] (ab15680)

製品の概要

  • 製品名Anti-MHC class I antibody [ERMP42]
    MHC class I 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [ERMP42] to MHC class I
  • 特異性Ab15680 detects murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd,H-2k,q,sp,r,w7,w22. MHC class I molecules of other haplotypes are not recognized by the antibody.
  • アプリケーション適用あり: IHC-P, IHC-Fr, Flow Cytmore details
  • 種交差性
    交差種: Mouse
    非交差種: Human
  • 免疫原

    murine macrophage precursor cells

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: BSA, PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified IgG from cell culture supernatant.
  • ポリ/モノモノクローナル
  • クローン名ERMP42
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/200. Acetone fixation recommended.
Flow Cyt 1/50 - 1/100.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • 細胞内局在Cell Membrane; Type I membrane protein.
  • 別名
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

Anti-MHC class I antibody [ERMP42] (ab15680) 使用論文

ab15680 has not yet been referenced specifically in any publications.

Product Wall



I contacted the laboratory to clarify the specificity of Anti-MHC class I antibody [ERMP42] (ab15680) for you.

I can confirm that the ab15680 does not react with MHC class I haplotypes in C57BL6 mice. I can recommend reviewing the da...

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ab15680 has indeed been tested in immunohistochemistry on paraffin slides. Although acetone fixation is recommended we have also obtained good results using paraformaldehyde as a fixative. In these cases, we suggest an antigen retrieval step using a ci...

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Thank you for your interesting question. We have some antibodies that recognize one species but not the other, but not all the targets for these antibodies are known to be expressed on these cell lines. What I found were two candidates:

ab15...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreatic tumor stroma cells)
Specification pancreatic tumor stroma cells
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

投稿 Oct 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Oct 04 2012

Thank you for your enquiry. This antibody was tested on human tonsil and skin but unfortunately, there isn't any staining on both tissues. Therefore, we have stated on the datasheet that this product does not react with human.

Thank you for your enquiry. I can confirm that indeed ab15680 will cross react with mouse and not human as it is very specific for the mouse protein. Please do not hesitate to contact us again if you require further details about ab15680,

Thank you for your enquiry, we have just received the following information: We have found in our lab that the antibody does not react on routinely fixed paraffin sections (4% formalin). However, from personal communications it seems that the antibo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"