製品の概要

  • 製品名Anti-MHC class I antibody [ER-HR 52]
    MHC class I 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [ER-HR 52] to MHC class I
  • 特異性Ab15681 detects MHC class I antigens of d and b haplotypes.
  • アプリケーション適用あり: IHC-Fr, IHC (PFA fixed)more details
    適用なし: IHC-FoFr
  • 種交差性
    交差種: Mouse
  • 免疫原

    Murine macrophage precursor cells.

  • 特記事項


    Ab15681 detects MHC class I antigens and is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA. pH 7.2
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考Ab15681 detects MHC class I antigens and is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.
  • ポリ/モノモノクローナル
  • クローン名ER-HR 52
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 1 µg/ml. The determinant recognised by ab15681 is glutaraldehyde (0.05%), paraformaldehyde (1%) and acetone-resistant.
IHC (PFA fixed) 1/300. PubMed: 17446933
  • 追加情報Is unsuitable for IHC-FoFr.
  • ターゲット情報

    • 関連性MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
    • 細胞内局在Cell Membrane; Type I membrane protein.
    • 別名
      • A 28 antibody
      • A 9 antibody
      • Antigen presenting molecule antibody
      • Aw 24 antibody
      • Aw 68 antibody
      • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
      • H2 K1 antibody
      • H2K antibody
      • HLA A antibody
      • HLA class I histocompatibility antigen A 1 alpha chain antibody
      • HLA class I histocompatibility antigen A 11 alpha chain antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A 24 alpha chain antibody
      • HLA class I histocompatibility antigen A 3 alpha chain antibody
      • HLA class I histocompatibility antigen A 30 alpha chain antibody
      • HLA class I histocompatibility antigen A 32 alpha chain antibody
      • HLA class I histocompatibility antigen A 68 alpha chain antibody
      • HLAA antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • MHC class I antigen A 1 antibody
      • MHC class I antigen A*11 antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A*24 antibody
      • MHC class I antigen A*3 antibody
      • MHC class I antigen A*30 antibody
      • MHC class I antigen A*32 antibody
      • MHC class I antigen A*68 antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC class I heavy chain H2 K antibody
      see all

    Anti-MHC class I antibody [ER-HR 52] 画像

    • Immunohistochemical analysis of frozen mouse brain tissue, post-infection with Salmonella typhimurium, staining MHC Class I with ab15681.
      Tissue was blocked with 2% BSA, 10% normal rabbit serum, before incubating with primary antibody overnight. A biotinylated anti-rat IgG was used as the secondary antibody. Staining was detected using DAB.

    Anti-MHC class I antibody [ER-HR 52] (ab15681) 使用論文

    This product has been referenced in:
    • Placke T  et al. Platelet-derived MHC class I confers a pseudonormal phenotype to cancer cells that subverts the antitumor reactivity of natural killer immune cells. Cancer Res 72:440-8 (2012). ICC/IF . Read more (PubMed: 22127925) »
    • Püntener U  et al. Long-term impact of systemic bacterial infection on the cerebral vasculature and microglia. J Neuroinflammation 9:146 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22738332) »

    See all 6 Publications for this product

    Product Wall

    Can you please tell me what kind of tissue you are trying to stain (species and organ), how long it was fixed in formalin, and if you included an antigen retrievals step before incubation with the antibody?

    The application that is listed on ...

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    My apologies for the late reply, I was away last week. I have received the following information from the source of ab15681: 1. Blocking endogenous biotin is important with the detection system used (biotinylated secondary). This may be improved. ...

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    I'm very sorry to hear you are having a problem with ab15681. This antibody has not been tested in IHC on perfusion formaldehyde fixed sections, we recommend using fresh frozen sections followed by postfixation in glutaraldehyde (0.05%), paraformaldehy...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"