Anti-MHC Class 1 H2 Db 抗体 [28-14-8] (Biotin) (ab25237)

製品の概要

  • 製品名Anti-MHC Class 1 H2 Db antibody [28-14-8] (Biotin)
    MHC Class 1 H2 Db 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [28-14-8] to MHC Class 1 H2 Db (Biotin)
  • 標識Biotin
  • 特異性This antibody binds to the a3 domain of H-2Db in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq.
  • アプリケーション適用あり: Flow Cyt, IHC-Frmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    C3H.SW mouse splenocytes.

  • エピトープThis antibody recognises an epitope in the a3 domain of H-2Db.
  • 特記事項


    This antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. This antibody has been shown to be useful in studies of complement mediated cytotoxicity.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.2% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • 一次抗体 備考This antibody has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells. This antibody has been shown to be useful in studies of complement mediated cytotoxicity.
  • ポリ/モノモノクローナル
  • クローン名28-14-8
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab18454-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 関連性In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • 細胞内局在Cell Membrane; single pass type I membrane protein
  • 参照データベース
  • 別名
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

Anti-MHC Class 1 H2 Db antibody [28-14-8] (Biotin) (ab25237) 使用論文

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I wouldn't recommend using ab128976 as an isotype control with ab25237 since ab128976 is raised in a goat and it needs to match the primary antibody you're using.

I would recommend ab18454 as an isotype...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"