Anti-MHC Class 1 H2 Db 抗体 [27-11-13] (FITC) (ab25028)

製品の概要

  • 製品名
    Anti-MHC Class 1 H2 Db antibody [27-11-13] (FITC)
    MHC Class 1 H2 Db 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db (FITC)
  • 由来種
    Mouse
  • 標識
    FITC. Ex: 493nm, Em: 528nm
  • 特異性
    ab25028 recognizes with the a3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the a3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • アプリケーション
    適用あり: Flow Cyt, IHC-Fr, Functional Studiesmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Mouse C3H.SW splenocytes

  • エピトープ
    An epitope on the a chain of H-2Db

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Affinity purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    27-11-13
  • アイソタイプ
    IgG2a
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab25028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. (Complement-dependent cytoxicity assay)

ターゲット情報

  • 関連性
    In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • 細胞内局在
    Cell Membrane; single pass type I membrane protein
  • 参照データベース
  • 別名
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

参考文献

ab25028 has not yet been referenced specifically in any publications.

レビューと Q&A

Both ab21321 and ab25028 recognize extracellular epitopes and can be used to stain live cells for FACS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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