製品の概要

  • 製品名Anti-MGST3 antibody [2G10CG7]
    MGST3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2G10CG7] to MGST3
  • アプリケーション適用あり: ICC/IF, In-Cell ELISA, IPmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    purified enzymes from bovine liver

  • ポジティブ・コントロール
    • ICC/IF: HDFn cells IP: Human and Rat liver lysates
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 2 µg/ml.
In-Cell ELISA Use a concentration of 2 µg/ml. (0.2 µg/well)
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Also functions as a glutathione peroxidase.
  • 組織特異性Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes.
  • 配列類似性Belongs to the MAPEG family.
  • 細胞内局在Endoplasmic reticulum membrane. Microsome membrane. Microsome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mgst3 antibody
    • MGST3_HUMAN antibody
    • Microsomal glutathione S transferase 3 antibody
    • Microsomal glutathione S-transferase 3 antibody
    • Microsomal GST-3 antibody
    • Microsomal GST-III antibody
    • Microsomal GST3 antibody
    • Microsomal GSTIII antibody
    see all

Anti-MGST3 antibody [2G10CG7] 画像

  • Immunocytochemistry image of ab110309-stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110309 at 2 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates primarily in the mitochondria.
  • Using MitoSciences' standard immunoprecipitation protocol, ab110309 pulls down the 15kDa MGST3 protein from human and rat liver samples. HepG2 lysate and mouse liver lysate yielded smaller amounts. This identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

Anti-MGST3 antibody [2G10CG7] (ab110309) 使用論文

ab110309 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"