製品の概要
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製品名Anti-MGST3 antibody [2G10CG7]
MGST3 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [2G10CG7] to MGST3
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アプリケーション適用あり: ICC/IF, In-Cell ELISA, IPmore details
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種交差性交差種: Rat, Human
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免疫原
purified enzymes from bovine liver
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ポジティブ･コントロール
- ICC/IF: HDFn cells IP: Human and Rat liver lysates
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特記事項
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
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バッファーPreservative: 0.02% Sodium azide
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Concentration information loading... -
精製度>95% by SDS-PAGE
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特記事項（精製）The purity of ab110309 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
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ポリ/モノモノクローナル
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クローン名2G10CG7
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アイソタイプIgG2a
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軽鎖の種類kappa
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab110309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 2 µg/ml. | |
| In-Cell ELISA | Use a concentration of 2 µg/ml. (0.2 µg/well) | |
| IP | Use at an assay dependent dilution. |
ターゲット情報
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機能Also functions as a glutathione peroxidase.
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組織特異性Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes.
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配列類似性Belongs to the MAPEG family.
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細胞内局在Endoplasmic reticulum membrane. Microsome membrane. Microsome membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 4259 Human
- Entrez Gene: 289197 Rat
- Omim: 604564 Human
- SwissProt: O14880 Human
- Unigene: 191734 Human
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別名
- Mgst3 antibody
- MGST3_HUMAN antibody
- Microsomal glutathione S transferase 3 antibody
see all
Anti-MGST3 antibody [2G10CG7] 画像
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![Immunocytochemistry/ Immunofluorescence - Anti-MGST3 antibody [2G10CG7 ] (ab110309)](http://a.abcam.com/ps/datasheet/images/110/ab110309/MGST3-Primary-antibodies-ab110309-3.gif)
Immunocytochemistry/ Immunofluorescence - Anti-MGST3 antibody [2G10CG7 ] (ab110309)Immunocytochemistry image of ab110309-stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110309 at 2 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates primarily in the mitochondria. -
![Immunoprecipitation - Anti-MGST3 antibody [2G10CG7 ] (ab110309)](http://a.abcam.com/ps/datasheet/images/110/ab110309/MGST3-Primary-antibodies-ab110309-4.gif)
Immunoprecipitation - Anti-MGST3 antibody [2G10CG7 ] (ab110309)Using MitoSciences' standard immunoprecipitation protocol, ab110309 pulls down the 15kDa MGST3 protein from human and rat liver samples. HepG2 lysate and mouse liver lysate yielded smaller amounts. This identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
Anti-MGST3 antibody [2G10CG7] (ab110309) 使用論文
ab110309 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"