Anti-MGST3 抗体 [2G10CG7] (ab110309)
Key features and details
- Mouse monoclonal [2G10CG7] to MGST3
- Suitable for: ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-MGST3 antibody [2G10CG7]
MGST3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2G10CG7] to MGST3 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IPmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: HDFn cells IP: Human and Rat liver lysates
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
The purity of ab110309 is near homogeneity as judged by SDS-PAGE (purity >95%). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
ポリ/モノ
モノクローナル -
クローン名
2G10CG7 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110309の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 2 µg/ml.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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ICC/IF
Use a concentration of 2 µg/ml. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Also functions as a glutathione peroxidase. -
組織特異性
Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes. -
配列類似性
Belongs to the MAPEG family. -
細胞内局在
Endoplasmic reticulum membrane. Microsome membrane. Microsome membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4259 Human
- Entrez Gene: 66447 Mouse
- Entrez Gene: 289197 Rat
- Omim: 604564 Human
- SwissProt: O14880 Human
- SwissProt: Q9CPU4 Mouse
- Unigene: 191734 Human
- Unigene: 218286 Mouse
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別名
- Mgst3 antibody
- MGST3_HUMAN antibody
- Microsomal glutathione S transferase 3 antibody
see all
画像
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Immunocytochemistry image of ab110309-stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110309 at 2 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates primarily in the mitochondria.
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Using MitoSciences' standard immunoprecipitation protocol, ab110309 pulls down the 15kDa MGST3 protein from human and rat liver samples. HepG2 lysate and mouse liver lysate yielded smaller amounts. This identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab110309 は論文での使用が確認できていません。