製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MGP
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Guinea pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 36-85 of Human MGP (

    NRRNANTFISPQQRWRAKVQERIRERSKPVHELNREACDDYRLCERYAMV

    ) NP_000891

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
  • 関連疾患
    Defects in MGP are the cause of Keutel syndrome (KS) [MIM:245150]. KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.
  • 配列類似性
    Belongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • 翻訳後修飾
    Requires vitamin K-dependent gamma-carboxylation for its function.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell growth inhibiting gene 36 protein antibody
    • Cell growth-inhibiting gene 36 protein antibody
    • GAMMA-CARBOXYGLUTAMIC ACID PROTEIN, MATRIX antibody
    • GIG36 antibody
    • Matrix Gla protein antibody
    • MGLAP antibody
    • MGP antibody
    • MGP_HUMAN antibody
    • NTI antibody
    see all

画像

  • Anti-MGP antibody (ab86233) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
    Additional bands at : 30 kDa,>60 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 10-20%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling MGP with ab86233 at 2.5µg/ml.

参考文献

ab86233 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Aorta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: NaCitrate 10mM
Permeabilization
No
Specification
Aorta
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 03 2015

Thank you for contacting us.

The anti-MGP antibody (ab70193) has been raised against the full length MGP protein and as it is a polyclonal antibody the exact epitope cannot be determined.We would expect theantibodyto be able to detectboth for...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録