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RabMAb

Anti-MGMT 抗体 [EPR4397] (ab108630)

製品の概要

  • 製品名
    Anti-MGMT antibody [EPR4397]
    MGMT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4397] to MGMT
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human MGMT.

  • ポジティブ・コントロール
    • WB: MCF7, Jurkat, HeLa, and HT29 cell lysates IHC-P: Hman clear cell carcinoma and spleen tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能
      Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
    • 配列類似性
      Belongs to the MGMT family.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 6 O methylguanine DNA methyltransferase antibody
      • 6-O-methylguanine-DNA methyltransferase antibody
      • Agat antibody
      • AGT antibody
      • AI267024 antibody
      • EC 2.1.1.63 antibody
      • Methylated DNA protein cysteine methyltransferase antibody
      • Methylated-DNA--protein-cysteine methyltransferase antibody
      • Methylguanine DNA methyltransferase antibody
      • MGC107020 antibody
      • MGMT antibody
      • MGMT_HUMAN antibody
      • O 6 methylguanine DNA alkyltransferase antibody
      • O 6 methylguanine DNA methyltransferase antibody
      • O-6-methylguanine-DNA methyltransferase antibody
      • O-6-methylguanine-DNA-alkyltransferase antibody
      see all

    Anti-MGMT antibody [EPR4397] 画像



    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MGMT knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108630 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108630 was shown to specifically react with MGMT when MGMT knockout samples were used. Wild-type and MGMT knockout samples were subjected to SDS-PAGE. ab108630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-MGMT antibody [EPR4397] (ab108630) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat ell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 22 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human clear cell carcinoma tissue by Immunohistochemistry.
    • ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human spleen tissue by Immunohistochemistry.

    Anti-MGMT antibody [EPR4397] (ab108630) 使用論文

    ab108630 has not yet been referenced specifically in any publications.

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    Leider ist es so, wie ich gestern schon vermutete: Wir haben die Antikörper- Konzentration in dem Zellkultur- Überstand leider nicht bestimmt.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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