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RabMAb

Anti-MGMT 抗体 [EPR4397] (ab108630)

製品の概要

  • 製品名
    Anti-MGMT antibody [EPR4397]
    MGMT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4397] to MGMT
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human MGMT aa 1-100. The exact sequence is proprietary.
    Database link: P16455

  • ポジティブ・コントロール
    • WB: MCF7, Jurkat, HeLa, and HT29 cell lysates IHC-P: Hman clear cell carcinoma and spleen tissues
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能
      Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
    • 配列類似性
      Belongs to the MGMT family.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 6 O methylguanine DNA methyltransferase antibody
      • 6-O-methylguanine-DNA methyltransferase antibody
      • Agat antibody
      • AGT antibody
      • AI267024 antibody
      • EC 2.1.1.63 antibody
      • Methylated DNA protein cysteine methyltransferase antibody
      • Methylated-DNA--protein-cysteine methyltransferase antibody
      • Methylguanine DNA methyltransferase antibody
      • MGC107020 antibody
      • MGMT antibody
      • MGMT_HUMAN antibody
      • O 6 methylguanine DNA alkyltransferase antibody
      • O 6 methylguanine DNA methyltransferase antibody
      • O-6-methylguanine-DNA methyltransferase antibody
      • O-6-methylguanine-DNA-alkyltransferase antibody
      see all

    画像

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MGMT knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108630 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108630 was shown to specifically react with MGMT when MGMT knockout samples were used. Wild-type and MGMT knockout samples were subjected to SDS-PAGE. ab108630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-MGMT antibody [EPR4397] (ab108630) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat ell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 22 kDa
      Observed band size: 23 kDa (why is the actual band size different from the predicted?)

    • ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human clear cell carcinoma tissue by Immunohistochemistry.
    • ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human spleen tissue by Immunohistochemistry.

    参考文献

    This product has been referenced in:
    • Lai SW  et al. Differential Characterization of Temozolomide-Resistant Human Glioma Cells. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 29301329) »
    • Niittykoski M  et al. Immunohistochemical Characterization and Sensitivity to Human Adenovirus Serotypes 3, 5, and 11p of New Cell Lines Derived from Human Diffuse Grade II to IV Gliomas. Transl Oncol 10:772-779 (2017). Read more (PubMed: 28797937) »

    See all 2 Publications for this product

    レビューと Q&A

    Leider ist es so, wie ich gestern schon vermutete: Wir haben die Antikörper- Konzentration in dem Zellkultur- Überstand leider nicht bestimmt.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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