Anti-MGMT 抗体 [EPR4397] (ab108630)

製品の概要

  • 製品名Anti-MGMT antibody [EPR4397]
    MGMT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4397] to MGMT
  • アプリケーション適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human MGMT.

  • ポジティブ・コントロール
    • WB: MCF7, Jurkat, HeLa, and HT29 cell lysates IHC-P: Hman clear cell carcinoma and spleen tissues
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
    • 配列類似性Belongs to the MGMT family.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 6 O methylguanine DNA methyltransferase antibody
      • 6-O-methylguanine-DNA methyltransferase antibody
      • Agat antibody
      • AGT antibody
      • AI267024 antibody
      • EC 2.1.1.63 antibody
      • Methylated DNA protein cysteine methyltransferase antibody
      • Methylated-DNA--protein-cysteine methyltransferase antibody
      • Methylguanine DNA methyltransferase antibody
      • MGC107020 antibody
      • MGMT antibody
      • MGMT_HUMAN antibody
      • O 6 methylguanine DNA alkyltransferase antibody
      • O 6 methylguanine DNA methyltransferase antibody
      • O-6-methylguanine-DNA methyltransferase antibody
      • O-6-methylguanine-DNA-alkyltransferase antibody
      see all

    Anti-MGMT antibody [EPR4397] 画像



    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MGMT knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108630 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108630 was shown to specifically react with MGMT when MGMT knockout samples were used. Wild-type and MGMT knockout samples were subjected to SDS-PAGE. ab108630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-MGMT antibody [EPR4397] (ab108630) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat ell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 22 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human clear cell carcinoma tissue by Immunohistochemistry.
    • ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human spleen tissue by Immunohistochemistry.

    Anti-MGMT antibody [EPR4397] (ab108630) 使用論文

    ab108630 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"