製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MGEA5
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Horse, Cow, Dog, Pig, Macaque monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human MGEA5.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: U87mg; JEG3; PDX1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab105217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
    Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
  • 組織特異性
    Ubiquitous. Shows highest expression in the brain, placenta and pancreas.
  • 配列類似性
    Belongs to the glycosyl hydrolase 84 family.
  • 翻訳後修飾
    Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
  • 細胞内局在
    Cytoplasm and Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Beta-hexosaminidase antibody
    • Beta-N-acetylglucosaminidase antibody
    • Beta-N-acetylhexosaminidase antibody
    • Bifunctional protein NCOAT antibody
    • HEXC antibody
    • Hyaluronidase in meningioma antibody
    • KIAA0679 antibody
    • MEA5 antibody
    • Meningioma expressed antigen 5 antibody
    • Meningioma-expressed antigen 5 antibody
    • MGEA 5 antibody
    • Mgea5 antibody
    • N-acetyl-beta-D-glucosaminidase antibody
    • N-acetyl-beta-glucosaminidase antibody
    • NCOAT antibody
    • Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody
    • Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody
    • OGA antibody
    • OGA_HUMAN antibody
    • Protein O-GlcNAcase antibody
    see all

画像

  • All lanes : Anti-MGEA5 antibody (ab105217) at 1 µg/ml

    Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
    Lane 3 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate
    Lane 4 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 5 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 6 : PDX1(Pancreatic and duodenal homeobox 1) Whole Cell Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 103 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ab105217 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab105217 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab105217 has not yet been referenced specifically in any publications.

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