製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to MGAT5
  • アプリケーション
    適用あり: WB, ELISA, Flow Cytmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Hamster
  • 免疫原

    Recombinant fragment: LAEPGQSCKQ VCQESQLICE PSFFQHLNKD KDMLKYKVTC QSSELAKDIL VPSFDPKNK HCVFQGDLLL FSCAGAHPRH QRVCPCRDFI KGQVALCKD, corresponding to amino acids 642 - 739 of Human MGAT5 (NP_002401) with a proprietary tag

  • ポジティブ・コントロール
    • Jurkat cell lysate
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 配列類似性
    Belongs to the glycosyltransferase 18 family.
  • 細胞内局在
    Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Alpha 1,3(6) mannosylglycoprotein antibody
    • Alpha 1,6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase antibody
    • Alpha mannoside beta 1,6 N acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha-mannoside beta-1 antibody
    • Beta 1,6 N acetyl glucosaminyltransferase antibody
    • GGNT5 antibody
    • GlcNAc T V antibody
    • GlcNAc-T V antibody
    • GNT V antibody
    • GNT VA antibody
    • GNT-V antibody
    • GNTV antibody
    • GNTVA antibody
    • Mannoside acetylglucosaminyltransferase 5 antibody
    • Mannosyl (alpha 1,6) glycoprotein beta 1,6 N acetyl glucosaminyltransferase antibody
    • Mgat5 antibody
    • MGT5A_HUMAN antibody
    • N acetylglucosaminyl transferase V antibody
    • N acetylglucosaminyltransferase V mannosyl (alpha 1,6) glycoprotein antibody
    • N-acetylglucosaminyl-transferase V antibody
    see all

画像

  • Anti-MGAT5 antibody (ab87977) at 1/2000 dilution + Human RWPE prostate cells at 20 µg

    Secondary
    Donkey anti-mouse polyclonal HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Additional bands at : 55 kDa (possible non-specific binding),95 kDa (possible post-translational modification).

    Exposure time : 40 seconds

    Image is courtesy of an AbReview submitted by Armen Petrosyan.

    Blocking was via 5% milk in PBST incubated for 1 hour at 22°C

    See Abreview

  • Overlay histogram showing Jurkat cells stained with ab87977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87977, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-MGAT5 antibody (ab87977) at 1 µg/ml + recombinant protein fragment with a proprietary tag (the immunogen) at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 85 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    The recombinant protein used as the immunogen has a predicted size of 37 kDa.
  • Anti-MGAT5 antibody (ab87977) at 1 µg/ml + jurkat cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 85 kDa

参考文献

ab87977 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 27 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (RWPE - prostate cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
20 µg
Specification
RWPE - prostate cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Oct 09 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PC-3 prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100
Specification
PC-3 prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Aug 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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