Anti-MGAT5 抗体 (ab68595)

製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MGAT5
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Sheep, Horse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human MGAT5.

    (Peptide available as ab68594.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Whole Cell Lysates: TE 671, Jurkat, Jurkat - Staurosporine Treated, MEF1 (data not shown) and HeLa - Hydroxyurea Treated (48hr, 2uM) (data not shown).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 84 kDa).Can be blocked with Human MGAT5 peptide (ab68594).

ターゲット情報

  • 機能
    Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
  • パスウェイ
    Protein modification; protein glycosylation.
  • 配列類似性
    Belongs to the glycosyltransferase 18 family.
  • 細胞内局在
    Golgi apparatus membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Alpha 1,3(6) mannosylglycoprotein antibody
    • Alpha 1,6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase antibody
    • Alpha mannoside beta 1,6 N acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha-mannoside beta-1 antibody
    • Beta 1,6 N acetyl glucosaminyltransferase antibody
    • GGNT5 antibody
    • GlcNAc T V antibody
    • GlcNAc-T V antibody
    • GNT V antibody
    • GNT VA antibody
    • GNT-V antibody
    • GNTV antibody
    • GNTVA antibody
    • Mannoside acetylglucosaminyltransferase 5 antibody
    • Mannosyl (alpha 1,6) glycoprotein beta 1,6 N acetyl glucosaminyltransferase antibody
    • Mgat5 antibody
    • MGT5A_HUMAN antibody
    • N acetylglucosaminyl transferase V antibody
    • N acetylglucosaminyltransferase V mannosyl (alpha 1,6) glycoprotein antibody
    • N-acetylglucosaminyl-transferase V antibody
    see all

画像

  • All lanes : Anti-MGAT5 antibody (ab68595) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 17 kDa, 70 kDa. We are unsure as to the identity of these extra bands.



    MGAT5 has a predicted molecular weight of 84 kDa; however it is a novel protein that is predicted to be extensively glycosylated (SwissProt data). Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

プロトコール

参考文献

ab68595 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
25 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

投稿 Nov 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Mar 28 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Jan 24 2017

Thank you for contacting us. MEF1 cells were used to test for mouse reactivity in WB. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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