Anti-MGT4A 抗体 [EPR10034] (ab151750)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10034] to MGT4A
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MGT4A antibody [EPR10034]
MGT4A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10034] to MGT4A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IP, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human MGAT4A aa 150-250. The exact sequence is proprietary.
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ポジティブ・コントロール
- Human fetal heart, fetal kidney and HeLa lysates; Human choriocarcinoma and pancreas tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR10034 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab151750の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ICC/IF |
1/250 - 1/500.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ICC/IF
1/250 - 1/500. |
IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Involved in glucose transport by mediating SLC2A2/GLUT2 glycosylation, thereby controlling cell-surface expression of SLC2A2 in pancreatic beta cells. -
組織特異性
Expressed in pancreas, spleen, thymus, prostate, small intestine, peripheral blood leukocytes and lymph node. Strongly overexpressed in choriocarcinoma cancer cell lines. Down-regulated in pancreatic cancer. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the glycosyltransferase 54 family. -
細胞内局在
Secreted and Golgi apparatus membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 11320 Human
- Entrez Gene: 269181 Mouse
- Entrez Gene: 367252 Rat
- SwissProt: Q9UM21 Human
- SwissProt: Q812G0 Mouse
- SwissProt: Q5M854 Rat
- Unigene: 177576 Human
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別名
- 3-D-mannoside beta-1 antibody
- 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A soluble form antibody
- 4-N-acetylglucosaminyltransferase IVa antibody
see all
画像
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All lanes : Anti-MGT4A antibody [EPR10034] (ab151750) at 1/1000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : HeLa lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 62 kDa -
Immunohistochemical analysis of paraffin-embedded Human choriocarcinoma tissue labeling MGAT4A with ab151750 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling MGAT4A with ab151750 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent analysis of HeLa cells labeling MGAT4A with ab151750 at 1/250 dilution.
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Immunohistochemical analysis of paraffin embedded Human Prostate hyperplasia tissue using ab151750 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Lung carcinoma tissue using ab151750 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab151750 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151750 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab151750 は 1 報の論文で使用されています。
- Schleicher EM et al. Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors. PLoS Genet 16:e1009176 (2020). PubMed: 33137164