製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MGAT2
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Monkey, Zebrafish, Cynomolgus monkey, Macaque monkey
  • 免疫原

    A region within synthetic peptide: SIQLYPNEFP GSDPRDCPRD LPKNAALKLG CINAEYPDSF GHYREAKFSQ, corresponding to internal sequence amino acids 180-229 of Human MGAT2

  • ポジティブ・コントロール
    • Jurkat cell lysate. Human kidney tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 関連性
    MGAT2 is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in its gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain.
  • 細胞内局在
    Golgi Apparatus membrane; type II membrane protein
  • 参照データベース
  • 別名
    • Alpha 1,6 mannosyl glycoprotein 2 beta N acetylglucosaminyltransferase antibody
    • Beta 1,2 N acetylglucosaminyltransferase II antibody
    • CDG2A antibody
    • CDGS2 antibody
    • GlcNAc T II antibody
    • GLCNACTII antibody
    • GNT II antibody
    • GNT2 antibody
    • Mannoside acetylglucosaminyltransferase 2 antibody
    • N glycosyl oligosaccharide glycoprotein N acetylglucosaminyltransferase II antibody
    • UDP N acetylglucosamine:alpha 6 D mannoside beta 1,2 N acetylglucosaminyltransferase II antibody
    see all

画像

  • Anti-MGAT2 antibody (ab62526) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling MGAT2 with ab62526 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

参考文献

ab62526 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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