製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MGA
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
  • 免疫原

    Immungen corresponds to a region within amino acids 2450-2500 of Human MGA (NP_001074010.2)

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab101269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use at 2-5 µg/mg of lysate.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 関連性
      MGA contains 1 basic helix-loop-helix (bHLH) domain and is a possible transcription factor.
    • 細胞内局在
      Nuclear
    • 参照データベース
    • 別名
      • MAD5 antibody
      • MAX dimerization protein 5 antibody
      • MAX gene associated antibody
      • MAX gene associated protein antibody
      • Max interacting protein antibody
      • MGAM antibody
      • MXD5 antibody
      see all

    画像

    • Detection of Human MGA by Immunoprecipitation in 1mg HeLa whole cell lysate (20% of IP loaded) using ab101269 at 3µg/mg of lysate. A different anti MGA antibody was used for subsequent WB detection using Chemiluminescence with an exposure time of 10 seconds.

    プロトコール

    参考文献

    ab101269 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Epiblast)
    Specification
    Epiblast
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Hydrogen Peroxide, DMSO
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    Mr. Andy Washkowitz

    Verified customer

    投稿 Jan 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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