Anti-METTL8 抗体 (ab122273)
Key features and details
- Rabbit polyclonal to METTL8
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-METTL8 antibody -
製品の詳細
Rabbit polyclonal to METTL8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
antigen sequence, corresponding to amino acids 1-77 (MNMIWRNSIS CLRLGKVPHR YQSGYHPVAP LGSRILTDPA KVFEHNMWDH MQWSKEEEAA ARKKVKENSA VRVLLEE) of Human METTL8.
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ポジティブ・コントロール
- IHC: Human stomach tissue. ICC/IF: Human cell line A-431 WB: Human cell line HepG2
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 33 kDa. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Probable methyltransferase. -
配列類似性
Belongs to the methyltransferase superfamily. METL family. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 79828 Human
- SwissProt: Q9H825 Human
- Unigene: 135146 Human
- Unigene: 560775 Human
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別名
- tension induced inhibited protein antibody
- methyltransferase like 8 antibody
- methyltransferase like protein 8 antibody
see all
画像
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Anti-METTL8 antibody (ab122273) at 0.4 µg/ml + Human cell line HepG2
Predicted band size: 33 kDa -
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab122273 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab1222273, at 1/15 dilution, staining METTL8 in Paraffin-embedded Human stomach tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab122273 は 2 報の論文で使用されています。
- Bolatkan A et al. Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules. Int J Oncol 60:N/A (2022). PubMed: 34913069
- Xie S et al. miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway. iScience 24:103271 (2021). PubMed: 34761190