製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to METTL7A
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the N terminal region of human METTL7A, conjugated to KLH.

  • ポジティブ・コントロール
    • 293 cell lysates transiently transfected with the METTL7A gene. Human hepatocarcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    ab62969 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50 - 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 28 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

ターゲット情報

  • 機能
    Probable methyltransferase.
  • 配列類似性
    Belongs to the methyltransferase superfamily.
  • 細胞内局在
    Lipid droplet. Endoplasmic reticulum. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAM B antibody
    • AAMB antibody
    • DKFZP586A0522 antibody
    • MET7A_HUMAN antibody
    • Methyltransferase like 7A antibody
    • Methyltransferase-like protein 7A antibody
    • METTL 7A antibody
    • METTL7A antibody
    • METTL7A methlytransferase like 7A antibody
    • Protein AAM-B antibody
    • Protein AAMB antibody
    see all

画像

  • All lanes : Anti-METTL7A antibody (ab62969) at 1/50 dilution

    Lane 1 : 293 cell lysates nontransfected
    Lane 2 : 293 cell lysates transiently transfected with the METTL7A gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • ab62969, at 1/10 dilution, staining METTL7A in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by immunohistochemistry. ab62969 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

参考文献

ab62969 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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