製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to METTL14
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 35-84 of Human METTL14 (NP_066012).

  • ポジティブ・コントロール
    • NCI-H226 cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization.
  • 配列類似性
    Belongs to the MT-A70-like family.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA1627 antibody
    • MET14_HUMAN antibody
    • Methyltransferase like 14 antibody
    • Methyltransferase-like protein 14 antibody
    • METTL14 antibody
    • N6-adenosine-methyltransferase subunit METTL14 antibody
    • OTTHUMP00000219837 antibody
    see all

画像

  • Anti-METTL14 antibody (ab98166) at 1 µg/ml (in 5% skim milk / PBS buffer) + NCI-H226 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa

プロトコール

参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Sample
Pig Cell lysate - whole cell (Swine testicle cells (ST))
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
40 µg
Specification
Swine testicle cells (ST)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Mar 09 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human embryonic stem cells)
Specification
human embryonic stem cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
30 µg
Specification
human embryonic stem cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

投稿 Jan 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Treatment
siRNA towards METTL14 (Dharmacon)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12% gel)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Samantha Johnston

Verified customer

投稿 Feb 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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