Anti-METTL11A 抗体 (ab72660)
Key features and details
- Mouse polyclonal to METTL11A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-METTL11A antibody -
製品の詳細
Mouse polyclonal to METTL11A -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human METTL11A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human liver lysate METTL11A transfected 293T lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72660の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
ターゲット情報
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機能
Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala, Pro or Ser residue in the [Ala/Pro/Ser]-Pro-Lys motif. Mediates the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation. -
配列類似性
Belongs to the methyltransferase superfamily. NTM1 family. -
細胞内局在
Nucleus. Predominantly nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 28989 Human
- Omim: 613560 Human
- SwissProt: Q9BV86 Human
- Unigene: 522433 Human
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別名
- AD 003 antibody
- Alpha N-terminal protein methyltransferase 1A antibody
- C9orf32 antibody
see all
画像
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Anti-METTL11A antibody (ab72660) at 1/500 dilution + Human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
All lanes : Anti-METTL11A antibody (ab72660) at 1/500 dilution
Lane 1 : METTL11A transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (2)
ab72660 は 2 報の論文で使用されています。
- Bade D et al. Modulation of N-terminal methyltransferase 1 by an N6-methyladenosine-based epitranscriptomic mechanism. Biochem Biophys Res Commun 546:54-58 (2021). PubMed: 33561748
- Park JH et al. Disruption of nucleocytoplasmic trafficking as a cellular senescence driver. Exp Mol Med 53:1092-1108 (2021). PubMed: 34188179