Methylated DNA Quantification Kit (Colorimetric) (ab117128)

製品の概要

  • 製品名Methylated DNA Quantification Kit (Colorimetric)
    5-methylcytosine キット 製品一覧
  • 検出方法Colorimetric
  • サンプルの種類
    Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • アッセイタイプQuantitative
  • 検出感度
    >= 0.2 ng/well
  • 全工程の試験時間
    4h 00m
  • 種交差性
    交差種: Species independent
  • 製品の概要

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

    Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

    It can be paired to use with Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab117128 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    Methylated DNA Quantification Kit (Colorimetric) 画像

    • Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.

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    Methylated DNA Quantification Kit (Colorimetric) (ab117128) 使用論文

    This product has been referenced in:
    • Farias N  et al. The effects of folic acid on global DNA methylation and colonosphere formation in colon cancer cell lines. J Nutr Biochem 26:818-26 (2015). Functional Studies ; Human . Read more (PubMed: 25804133) »
    • Panikar CS  et al. Presence of DNA methyltransferase activity and CpC methylation in Drosophila melanogaster. Mol Biol Rep N/A:N/A (2015). Functional Studies ; Drosophila melanogaster . Read more (PubMed: 26547851) »

    See all 2 Publications for this product

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    I am very pleased to hear you would like to accept our offer and test ab117128 with "Flatworm species". This code will give you €350 off your next order before the expiration date. To redeem this offer, please submit an Abreview for...

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    Thank you for your reply.

    Tween at 0.05 - 0.1% should be used.

    I hope this information helps.

    Thank you for your inquiry.

    I heard back from the lab that generally, PBS-T buffer may be used for washing, however for the best results, they recommend to use the wash buffer included in the kit. We could get the washing buffer (either 14 mL...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"